This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Imahori, Y. Articles by Ueda, S. Search for Related Content PubMed PubMed Citation Articles by Imahori, Y. Articles by Ueda, S.

Rapid Incorporation of Carbon-11-labeled Diacylglycerol as a Probe of Signal Transduction in Glioma¹

Department of Neurosurgery, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo 602, Kyoto, Japan [Y. I., Y. O., K. M., S. U.], and Cyclotron Unit, Nishijin Hospital, Kamigyo 602, Kyoto, Japan [R. F.]

We have synthesized and characterized a positron-emitting carbon-11-labeled 1,2-diacylglycerol to study phosphoinositide turnover in tumor cells. Rapid incorporation of the 1,2-diacylglycerol was observed in the C6 glioma cell line. The incorporated lipid fraction consisted chiefly of phosphoinositide pool and another phospholipid pool in the proliferative state. When the state was inhibited by (-)-3D-3-deoxy-3-fluoro-myo-inositol, incorporation into the phosphoinositide pool decreased selectively. This suggested that phosphoinositide turnover is the leading regulator of tumor proliferation potential. On the basis of the concept of carbon-11-labeled 1,2-diacylglycerol as a specific probe for visualizing the tumor signal transduction in vivo, we obtained proliferating images of implanted C6 glioma cells in the rat brain by autoradiography and visualized the proliferation signal in human glioma by positron emission tomography.

¹ This work was supported in part by Grants-in-Aid for Science Research (02454337, 05671179, 06282254, 06671411, and 07274259) from the Ministry of Education Science and Culture of Japan. Animal experiments and care followed the Guidelines for the Care and Use of Laboratory Animals established by The Animal Care Committee of Kyoto Prefectural University of Medicine.

² To whom requests for reprints should be addressed.

Received 4/27/95. Accepted 8/17/95.

This article has been cited by other articles:

				H. Otani, Y. Kagaya, Y. Imahori, S. Yasuda, R. Fujii, M. Chida, S. Namiuchi, M. Takeda, M. Sakuma, J. Watanabe, et al. Myocardial 11C-Diacylglycerol Accumulation and Left Ventricular Remodeling in Patients After Myocardial Infarction J. Nucl. Med., April 1, 2005; 46(4): 553 - 559. [Abstract] [Full Text] [PDF]

				M. Chida, Y. Kagaya, Y. Imahori, S. Namiuchi, R. Fujii, M. Fukuchi, C. Takahashi, F. Tezuka, T. Ido, and K. Shirato Visualization of Myocardial Phosphoinositide Turnover with 1-[1-11C]-Butyryl-2-Palmitoyl-rac-Glycerol in Rats with Myocardial Infarction J. Nucl. Med., December 1, 2000; 41(12): 2063 - 2068. [Abstract] [Full Text] [PDF]