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Alteration of Glyceraldehyde-3-phosphate Dehydrogenase Activity and Messenger RNA Content by Androgen in Human Prostate Carcinoma Cells¹

University of Wisconsin Comprehensive Cancer Center [G. W.], Department of Medicine [G. W.], Environmental Toxicology Center [M. O. R., G. W.], and the William S. Middleton Veteran's Administration Hospital [G. W.], University of Wisconsin, Madison, Wisconsin 53792

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) activity and mRNA content is altered in the androgen-responsive human prostate carcinoma cell line LNCaP after exposure to the synthetic androgen R1881. Elevation in GAPDH activity is noted as early as 24 h after treatment with 1 nM R1881 and lasts at least 96 h. R1881 has no effect on the activity of GAPDH in androgen-independent DU145 cells. LNCaP GAPDH mRNA content is lowered by treatment with 1 nM R1881; the magnitude of reduction appears to depend on the length of exposure. The results present at least one means by which androgen-responsive tissues may develop alterations in GAPDH mRNA or activity, as is found in certain tumor tissues.

¹ Supported by Veteran's Administration, Environmental Toxicology Training Grant 5-T32-ES07015-18, CaP CURE.

² To whom requests for reprints should be addressed, at Medical Oncology Section, Department of Medicine, University of Wisconsin, K4/666 CSC, 600 Highland Avenue, Madison, WI 53792.

Received 6/26/95. Accepted 8/25/95.

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