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Detection of p53 Mutations in Benign and Dysplastic Nevi¹

Department of Biology, Centre for Environmental Health, University of Victoria, Victoria [D. B. L., G. V. d. A., J. W., W. K.]; British Columbia Cancer Agency, Victoria [K. W.]; and University of British Columbia, Vancouver [K. W., P. K.], British Columbia, Canada

We have examined melanocytic cells derived directly from fresh biopsy tissue for the presence of p53 mutations. Using selective media that permits growth of melanocytes and inhibits growth of fibroblasts and keratinocytes, we established short-term, primary cultures of melanocytes from skin biopsies of common acquired nevi, dysplastic nevi, and from metastatic melanoma. Using PCR-single-stranded conformational polymorphism analysis, we have detected p53 mutations in 2 of 11 benign compound nevi and 2 of 5 dysplastic nevi. All nevi positive for p53 mutations were derived from patients who previously had cutaneous moles and three of the four had a family and/or personal history of melanoma.

¹ Supported by the Natural Sciences and Engineering Council of Canada (NSERC) and Kabi Pharmacia by their contributions to the NSERC-Industrial Research partnership grant.

² To whom requests for reprints should be addressed, at Department of Biology, Centre for Environmental Health, University of Victoria, P.O. Box 1700, Victoria, British Columbia, V8W 2Y2 Canada.

Received 7/31/95. Accepted 8/18/95.

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