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Tumor Suppression by p21^WAF11

Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan 48201 [Y. Q. C., S. C. C., J. M. A.], and Michigan Cancer Foundation, Detroit, Michigan 48202 [F. R. M.]

The p21^WAF1 gene encodes a cyclin-dependent kinase inhibitor and mediates tumor suppressor gene p53-induced cell cycle arrest. To directly test whether p21^WAF1 can act as a tumor suppressor, we have expressed the p21^WAF1 cDNA in several human tumor cell lines using a tetracycline-inducible system. Overexpression of p21^WAF1 suppresses proliferation and soft agar growth of tumor cells in vitro, as well as tumorigenicity in vivo. Our data provide direct evidence for the tumor-suppressive activity of p21^WAF1.

¹ Supported in part by American Cancer Society Grant IN-162 and a fund from the Pathology Department, Wayne State University.

² To whom requests for reprints should be addressed, at Department of Pathology, Wayne State University, 540 East Canfield Avenue, Detroit, MI 48201. Phone: (313) 577-5634; Fax: (313) 577-0057; E-mail: yqchen@med.wayne.edu.

Received 6/27/95. Accepted 8/31/95.

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