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An Integrated High-Resolution Physical Map of the DPC/BRCA2 Region at Chromosome 13q12¹

Departments of Pathology [M. S., E. R., C. A. M., A. T. M. S. H., S. A. H., L. T. d. C., S. E. K.] and Oncology [S. E. K.], The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205-2196, and Human Genetics Department, Roswell Park Cancer Institute, Buffalo, New York 14263 [X. G., P. J. d. J.].

We identified a homozygous deletion in a pancreatic carcinoma (DPC) that localized to a 1-cM region at chromosome 13q12.3, which lay within the 6-cM locus of familial breast cancer susceptibility (BRCA2). Here we present a physical map of the region, consisting of YAC, PAC, and cosmid contigs. The YAC contig comprises 16 clones that together span the entire BRCA2 region. The PAC contig comprises 22 clones that together span the DPC region. Seventy cosmid clones were localized within and near the DPC region. Thirty-five sequence-tagged sites were defined and localized within the map. The map indicates the size of the DPC region to be near 250 kb, and provides mapped and cloned resources for the search for the putative tumor suppressor gene(s) in the region.

¹ This work was supported by the SPORE in Gastrointestinal Cancer, NIH Grant CA62924, by the Dutch Cancer Society KWF (M. S.), by the Deutschen Krebshilfe (S. A. H.), and by JNICT Scholarship BD1508/91-ID (L. T. d. C.). X. G. and P. J. d. J. are supported by Cancer Center Support Grant CA-16056 at Roswell Park Cancer Institute, by Department of Energy Grant DE-FG02-94ER61883, and by NIH Grant IR01RG01165-01. S. E. K. is a McDonnell Foundation Scholar.

² To whom requests for reprints should be addressed, at Department of Oncology, The Johns Hopkins Medical Institutions, Baltimore, MD 21205-2196. Phone: (410) 614-3314; Fax: (410) 614-0675.

Received 8/22/95. Accepted 9/ 7/95.

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