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Regression of Established Tumors in Mice Mediated by the Oral Administration of a Recombinant Listeria monocytogenes Vaccine¹

Department of Microbiology and the University of Pennsylvania Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6076 [Z-K. P., G. I., Y. P.] and Department of Oncology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 [D. P.]

We have shown previously that Listeria monocytogenes, a gram-positive, facultative intracellular bacterium, is a potent vector for targeting tumor-specific antigens to the immune system. After parenteral administration, we observed protection against both renal and colorectal mouse tumors and regression of established renal tumors. In the present study, we have exploited the fact that the normal route of infection of this organism is through the gut. We show that an L. monocytogenes recombinant that expresses a model tumor antigen is an effective cancer immunotherapeutic agent when delivered orally in that it causes regression of established, macroscopic mouse renal and colorectal tumors expressing the same antigen.

¹ Supported in part by NIH Grant GM31841 to Y. P., who is also the recipient of an ACS Faculty Research Award.

² To whom requests for reprints should be addressed, at University of Pennsylvania Medical Center, Department of Microbiology, 209 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104-6076.

Received 8/ 7/95. Accepted 9/18/95.

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