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Absence of Secretory Phospholipase A₂ Gene Alterations in Human Colorectal Cancer¹

The Johns Hopkins Oncology Center, Baltimore, Maryland 21231 [G. J. R., B. V., K. W. K.]; Department of Medicine, Ireland Clinical Center, University Hospitals of Cleveland and Case Western Reserve University, Cleveland, Ohio 44106 [S. M., J. K. W.]; and Howard Hughes Medical Institute, Baltimore, Maryland 21231 [B. V.]

A potent modifying locus of intestinal tumorigenesis in the mouse was recently identified as secretory phospholipase A₂ (sPLA₂). The human homologue of sPLA₂ maps to chromosome 1p35, a region frequently lost in human tumors. To evaluate the possibility that sPLA₂ was a tumor suppressor gene that was the target of the 1p loss events, we identified polymorphisms within the human sPLA₂ gene. Using these polymorphisms, 31% of 16 colorectal carcinomas were found to lose a sPLA₂ allele. However, sequence analysis of the complete coding region of sPLA₂ revealed no somatic mutations in the remaining allele of those tumors with allelic loss, nor in 18 additional colorectal cancers. Thus, sPLA₂ is within the chromosomal region often lost during colorectal tumorigenesis, but mutations of this gene do not appear to play a major role in colorectal cancer development, and sPLA₂ is unlikely to be the 1p35 tumor suppressor.

¹ This work was supported by NIH Grants CA57345 and CA09243.

² To whom requests for reprints should be addressed, at The Johns Hopkins Oncology Center, 424 North Bond Street, Baltimore, MD 21231.

Received 9/ 7/95. Accepted 10/ 2/95.

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