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Lack of Correlation between DNA-dependent Protein Kinase Activity and Tumor Cell Radiosensitivity¹

Radiobiology [M. J. A-T., G. M. B.] and Molecular Oncology Programs [R. S. D.], Cross Cancer Institute, Edmonton, Alberta T6G 1Z2, and Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4 [L. G. L., S. P. L-M.], Canada

Lack of DNA-dependent protein kinase (DNA-PK) activity confers radiosensitivity and defective DNA double-strand break repair. Nine human malignant glioma cell lines were studied to determine whether differences in DNA-PK activity reflect differences in inherent radiosensitivity or are predictive of tumor treatment response. DNA-PK activity was present in all cell extracts, as were the DNA-PK proteins, DNA-PK catalytic subunit, Ku p70, and Ku p80. No correlation was found between the levels of DNA-PK activity and inherent radiosensitivity or in the tumor treatment response. These preliminary results suggest that variation in DNA-PK activity may not be a determinant of clinical response in malignant glioma.

¹ This work was supported by awards from the National Cancer Institute of Canada with funds provided by the Canadian Cancer Society, the Natural Sciences and Engineering Research Council (NSERC) (Canada), and the Alberta Cancer Board. S. P. L-M. is the recipient of an NSERC Women's Faculty Award.

² To whom requests for reprints should be addressed, at Radiobiology, Cross Cancer Institute, 11560 University Avenue, Edmonton, Alberta T6G 1Z2, Canada.

Received 8/23/95. Accepted 10/ 4/95.

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