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Ornithine Decarboxylase Transformation of NIH/3T3 Cells Is Mediated by Altered Epidermal Growth Factor Receptor Activity¹

Departments of Internal Medicine, Division of Gastroenterology [J. A. M., E. M-P., J. T., J. D., M. S., A. P. N. M.], and The Center for Molecular Medicine and Genetics [J. A. M., H. G.], Wayne State University School of Medicine, Detroit, Michigan 48201, and Research Service, Veterans Administration Medical Center, Allen Park, Michigan 48101 [E. M-P., J. T., A. P. N. M.]

Ornithine decarboxylase (ODC) has been shown to be oncogenic in transfected NIH/3T3 cells overexpressing the enzyme from a heterologous promoter. These cells, designated as NODC-2 cells, acquire proliferative properties associated with tumorigenic transformation such as loss of contact inhibition, decreased population doubling time, anchorage-independent growth, and tumor production in nude mice. At least one of these parameters, loss of contact inhibition, remains dependent on elevated ODC levels. We have used these cells to investigate the molecular mechanisms by which ODC overexpression drives cell transformation and to examine the involvement of other proto-oncogene products in this process. An interaction between ODC overexpression and the epidermal growth factor receptor (EGF-R) was suggested initially by the elevation of both basal (300%) and ligand-induced (457%) EGF-R tyrosine kinase activities in NODC-2 cells compared to similarly treated control NLK cells. Disruption of EGF-R mediated signal transduction in NODC-2 cells both by treatment with tyrphostin-25 or by transfection with a vector expressing a dominant negative EGF-R mutant resulted in reacquisition of contact-inhibited growth and suppression of anchorage-independent, clonogenic growth in soft agar. We conclude that ODC-induced transformation of NIH/3T3 cells is mediated, at least partly, by alterations in EGF-R signal transduction activity.

¹ This work was supported in part by funds from The Department of Internal Medicine and The Fund for Medical Research, Wayne State University School of Medicine (J. A. M.), by NIH/National Institute of Aging Grant AG08438 (A. P. N. M.), and by grants from the Department of Veterans Affairs (A. P. N. M.).

² To whom requests for reprints should be addressed, at Department of Internal Medicine, Wayne State University School of Medicine, 540 East Canfield, Detroit, MI 48201.

Received 6/ 2/95. Accepted 9/18/95.

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