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Apoptin, a Protein Derived from Chicken Anemia Virus, Induces p53-independent Apoptosis in Human Osteosarcoma Cells¹

Laboratory of Molecular Carcinogenesis, Sylvius Laboratories, Leiden University, P.O. Box 9503, 2300 RA Leiden, the Netherlands

Previously, we have shown that the chicken anemia virus-derived VP3 ("apoptin") protein induces apoptosis in chicken mononuclear cells. Here, we report that apoptin also induces apoptosis in human osteosarcoma cells, regardless of whether they expressed wild-type, mutant p53, or no p53 at all. Moreover, the nuclear location of apoptin appears to be important for its optimal induction of apoptosis. The fact that apoptin can induce p53-independent apoptosis in human tumor cells makes apoptin a potential candidate for treatment of frequently occurring types of cancer cells that do not contain functional p53.

¹ This research was partially made possible by grants from the Netherlands Ministry of Economic Affairs and Aesculaap BV, Boxtel, the Netherlands.

² To whom requests for reprints should be addressed.

Received 11/29/94. Accepted 12/16/94.

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