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Natural Estrogens Induce Modulation of Microtubules in Chinese Hamster V79 Cells in Culture

Division of Biochemistry, Kyoritsu College of Pharmacy, Shibakoen 1-chome, Minato-ku, Tokyo 105, Japan

Natural estrogens and their derivatives comprising 30 compounds in total were tested for their ability to induce microtubule disruption in Chinese hamster V79 cells. The cytoplasmic microtubule networks were examined by the indirect immunofluorescence method using anti-ß-tubulin antibody. The effective concentrations of 17ß-estradiol (E₂-17ß) and 17-estradiol required for the induction of microtubule distribution in 50% of the cells (EC₅₀) in 1 h were 10 and 9 µM for V79 cells, respectively, and 2-methoxyestradiol showed the strongest activity (EC₅₀ 2 µM) among the tested compounds including the catechol estrogens 2-hydroxylestradiol, 4-hydroxyestradiol, 2-hydroxyestrone, and 4-hydroxyestrone. The estrone series of estrogens showed relatively low activity for disruption of micro-tubule networks compared with E₂-17ß, whereas 3-deoxy-3-methylestradiol showed almost the same EC₅₀ value as E₂-17ß. There was a correlation between the EC₅₀ values of the compounds and their growth-inhibitory activities. The presence of 1 µM taxol in culture protected against 50 µM E₂-17ß-induced microtubule disruption. Cycloheximide and actinomycin D had no preventive action on the effect of E₂-17ß, suggesting that the microtubule-disruptive effect of E₂-17ß is not associated with newly synthesized proteins and mRNAs. The present results indicate that some natural estrogens cause microtubule disruption in a nongenomic manner.

¹ To whom requests for reprints should be addressed.

Received 11/21/94. Accepted 3/ 1/95.

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