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Cloning and Characterization of TCTA, a Gene Located at the Site of a t(1;3) Translocation¹

Departments of Pediatrics and Molecular Medicine, Roswell Park Cancer Institute, Buffalo, New York 14263 [P. D. A., D. S. C.]; Division of Hematology/Oncology, Children's Hospital of Buffalo, Buffalo, New York 14263 [P. D. A.]; and National Cancer Institute, Navy Medical Oncology Branch, Bethesda, Maryland 20899-5105 [B. E. J., E. R., I. R. K.]

We have cloned and characterized a novel gene at the site of a t(1;3)(p34;p21) translocation breakpoint in T-cell acute lymphoblastic leukemia. A cDNA for this gene, for which we propose the designation TCTA (T-cell leukemia translocation-associated gene), has been cloned. TCTA mRNA is expressed ubiquitously in normal tissues, with the highest levels of expression seen in the kidney. The TCTA gene is conserved throughout evolution in organisms ranging from Drosophila to humans. A short open reading frame encodes a predicted M_r 12,000 protein without strong homology to any previously reported proteins. Of note, genomic Southern blots demonstrated a reduced TCTA signal in three of four small cell lung cancer cell lines tested, suggesting loss of one of the two copies of the gene.

¹ This work was supported in part by grants (to P. D. A.) from the Roswell Park Alliance Foundation and the Association for Research of Childhood Cancer.

² To whom requests for reprints should be addressed, at Department of Pediatrics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263.

Received 10/10/94. Accepted 3/ 6/95.

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