This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fernandez-Madrid, F. Articles by Ager, J. W. Search for Related Content PubMed PubMed Citation Articles by Fernandez-Madrid, F. Articles by Ager, J. W.

Autoimmunity to Collagen in Human Lung Cancer¹

Wayne State University School of Medicine, Department of Internal Medicine, Division of Rheumatology [F. F-M., R. L. K., B. C.], Division of Oncology [M. J. K.], and School of Science, Department of Psychology [J. W. A.], Detroit, Michigan 48201

Autoantibodies have been described in human cancer patients as well as in animal models of malignancy. The extracellular matrix and especially basement membranes act as barriers for tumor cell invasion. Collagen, particularly types I, III, and IV, are major constituents of the extracellular matrix. We tested the hypothesis that autoimmunity to collagen antigens is present in lung cancer. Sera from 67 patients with lung cancer and 50 reference subjects were tested for anticollagen antibodies by using purified human collagen types I-V and for antibodies binding human cartilage aggrecan proteoglycan. Antibody levels were determined by using ELISA. The relationship of serum levels of these antibodies to patient survival, histology, treatment response, disease stage, and pack years of smoking was examined by using multiple regression and discriminant function analyses. A subgroup of 45 patients in whom a smoking history was available was analyzed separately. Within 1 month of the initiation of therapy, mean serum levels of antibodies binding fibrillar collagen types I-III and V were significantly higher (P < 0.025) than were those in control sera (43.2% of patients positive for one or more anticollagen antibodies). Antibodies binding aggrecan proteoglycan were not different between patients and control sera. In the lung cancer patients, the levels of serum antibodies binding types IV and V collagens contributed to the variance of progression-free survival days, survival days, and the duration of favorable response in opposite directions. Histological cell type contributed to the variance in the level of serum antibody binding collagen types IV and V. Lower levels of antibody binding type IV and higher levels of antibody binding type V were associated with small cell carcinoma. The pack-years of smoking only contributed to the variance in the level of serum antibody binding type V collagen. We conclude that autoantibodies to fibrillar collagen antigens are present frequently in lung cancer patients, and their levels may be related to histological cell type and to the duration of the response to treatment.

¹ This work was supported in part by Hutzel Hospital and the Flora Temple Fund.

² To whom requests for reprints should be addressed, at Center For Rheumatic Diseases, Hutzel Hospital, 4707 St. Antoine, Lobby Center, Detroit, MI 48201.

Received 7/31/95. Accepted 10/31/95.

This article has been cited by other articles:

				F. Fernandez-Madrid, P. J. VandeVord, X. Yang, R. L. Karvonen, P. M. Simpson, M. J. Kraut, J. L. Granda, and J. E. Tomkiel Antinuclear Antibodies as Potential Markers of Lung Cancer Clin. Cancer Res., June 1, 1999; 5(6): 1393 - 1400. [Abstract] [Full Text] [PDF]