This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Candidus, S. Articles by Höfler, H. Search for Related Content PubMed PubMed Citation Articles by Candidus, S. Articles by Höfler, H.

No Evidence for Mutations in the - and ß-Catenin Genes in Human Gastric and Breast Carcinomas

GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Pathologie, Neuherberg, Postfach 11 29, D-85758 Oberschleissheim [S. C., P. B., K-F. B., H. H.], and Institut für Pathologie der Technischen Universität, Klinikum rechts der Isar, Ismaningerstrasse 22, D-81675 München [H. H.], Germany

Disturbed function of E-cadherin and/or of one of its anchoring proteins, the catenins, is thought to destabilize E-cadherin-mediated cell-cell adhesion, which may enhance the invasiveness of epithelial cells and thus favor carcinoma progression. Reduced expression of E-cadherin and -catenin, as well as mutations in the E-cadherin gene, have been found in various carcinomas, whereas mutations in the - and ß-catenin genes have been described only in carcinoma cell lines. Using reverse transcription-PCR, followed by agarose gel electrophoresis and single-strand conformational polymorphism, we examined 16 diffuse- and 5 intestinal-type gastric carcinomas, as well as 9 lobular and 2 ductal breast carcinomas, for mutations of - and ß-catenin cDNA. All of the investigated tumors were analyzed previously for E-cadherin mutations. Comparing tumorous and nontumorous samples, we detected neither deletions nor aberrant single-strand conformational polymorphism patterns. At nucleotide 2220 of the -catenin gene, we identified one frequent polymorphism. Our findings suggest that, in contrast to E-cadherin, mutations of - and ß-catenin do not contribute to the pathogenesis or the diffuse growth patterns of gastric or breast carcinomas.

¹ To whom requests for reprints should be addressed.

Received 9/18/95. Accepted 11/14/95.

This article has been cited by other articles:

				M. J. Hayes, D. Thomas, A. Emmons, T. J. Giordano, and C. G. Kleer Genetic Changes of Wnt Pathway Genes Are Common Events in Metaplastic Carcinomas of the Breast Clin. Cancer Res., July 1, 2008; 14(13): 4038 - 4044. [Abstract] [Full Text] [PDF]

				M. P.V. Shekhar, B. Gerard, R. J. Pauley, B. O. Williams, and L. Tait Rad6B Is a Positive Regulator of {beta}-Catenin Stabilization Cancer Res., March 15, 2008; 68(6): 1741 - 1750. [Abstract] [Full Text] [PDF]

				M. P.V. Shekhar, L. Tait, and B. Gerard Essential Role of T-Cell Factor/{beta}-Catenin in Regulation of Rad6B: A Potential Mechanism for Rad6B Overexpression in Breast Cancer Cells Mol. Cancer Res., October 1, 2006; 4(10): 729 - 745. [Abstract] [Full Text] [PDF]

				M. Dolled-Filhart, A. McCabe, J. Giltnane, M. Cregger, R. L. Camp, and D. L. Rimm Quantitative In situ Analysis of {beta}-Catenin Expression in Breast Cancer Shows Decreased Expression Is Associated with Poor Outcome. Cancer Res., May 15, 2006; 66(10): 5487 - 5494. [Abstract] [Full Text] [PDF]

				J. Rios-Doria, R. Kuefer, S. P. Ethier, and M. L. Day Cleavage of {beta}-Catenin by Calpain in Prostate and Mammary Tumor Cells Cancer Res., October 15, 2004; 64(20): 7237 - 7240. [Abstract] [Full Text] [PDF]

				W. Li, W. Qiao, L. Chen, X. Xu, X. Yang, D. Li, C. Li, S. G. Brodie, M. M. Meguid, L. Hennighausen, et al. Squamous cell carcinoma and mammary abscess formation through squamous metaplasia in Smad4/Dpc4 conditional knockout mice Development, December 15, 2003; 130(24): 6143 - 6153. [Abstract] [Full Text] [PDF]

				M. P.A. Ebert, J. Yu, J. Hoffmann, A. Rocco, C. Rocken, S. Kahmann, O. Muller, M. Korc, J. J. Sung, and P. Malfertheiner Loss of Beta-Catenin Expression in Metastatic Gastric Cancer J. Clin. Oncol., May 1, 2003; 21(9): 1708 - 1714. [Abstract] [Full Text] [PDF]

				M. Mareel and A. Leroy Clinical, Cellular, and Molecular Aspects of Cancer Invasion Physiol Rev, April 1, 2003; 83(2): 337 - 376. [Abstract] [Full Text] [PDF]

				S. Nabais, J. C. Machado, C. Lopes, R. Seruca, F. Carneiro, and M. Sobrinho-Simoes Patterns of {beta}-Catenin Expression in Gastric Carcinoma: Clinicopathological Relevance and Mutation Analysis International Journal of Surgical Pathology, January 1, 2003; 11(1): 1 - 9. [Abstract] [PDF]

				W. M. Clements, J. Wang, A. Sarnaik, O. J. Kim, J. MacDonald, C. Fenoglio-Preiser, J. Groden, and A. M. Lowy {beta}-Catenin Mutation Is a Frequent Cause of Wnt Pathway Activation in Gastric Cancer Cancer Res., June 1, 2002; 62(12): 3503 - 3506. [Abstract] [Full Text] [PDF]

				M. P.A. Ebert, G. Fei, S. Kahmann, O. Muller, J. Yu, J. J.Y. Sung, and P. Malfertheiner Increased {beta}-catenin mRNA levels and mutational alterations of the APC and {beta}-catenin gene are present in intestinal-type gastric cancer Carcinogenesis, January 1, 2002; 23(1): 87 - 91. [Abstract] [Full Text] [PDF]

				D. Liu, C.-l. Huang, K. Kameyama, E. Hayashi, A. Yamauchi, S. Kobayashi, and H. Yokomise E-cadherin expression associated with differentiation and prognosis in patients with non-small cell lung cancer Ann. Thorac. Surg., March 1, 2001; 71(3): 949 - 954. [Abstract] [Full Text] [PDF]

				P. W. Schlosshauer, S. A. Brown, K. Eisinger, Q. Yan, E. R. Guglielminetti, R. Parsons, L. H. Ellenson, and J. Kitajewski APC truncation and increased {beta}-catenin levels in a human breast cancer cell line Carcinogenesis, July 1, 2000; 21(7): 1453 - 1456. [Abstract] [Full Text] [PDF]

				W. S. Park, R. R. Oh, J. Y. Park, S. H. Lee, M. S. Shin, Y. S. Kim, S. Y. Kim, H. K. Lee, P. J. Kim, S. T. Oh, et al. Frequent Somatic Mutations of the {beta}-Catenin Gene in Intestinal-Type Gastric Cancer Cancer Res., September 1, 1999; 59(17): 4257 - 4260. [Abstract] [Full Text] [PDF]

				C. Gamallo, J. Palacios, G. Moreno, J. Calvo de Mora, A. Suarez, and A. Armas {beta}-Catenin Expression Pattern in Stage I and II Ovarian Carcinomas : Relationship with {beta}-Catenin Gene Mutations,Clinicopathological Features, and Clinical Outcome Am. J. Pathol., August 1, 1999; 155(2): 527 - 536. [Abstract] [Full Text] [PDF]

				K. Caca, F. T. Kolligs, X. Ji, M. Hayes, J.-m. Qian, A. Yahanda, D. L. Rimm, J. Costa, and E. R. Fearon {beta}- and {{gamma}}-Catenin Mutations, but not E-Cadherin Inactivation, Underlie T-Cell Factor/Lymphoid Enhancer Factor Transcriptional Deregulation in Gastric and Pancreatic Cancer Cell Growth Differ., June 1, 1999; 10(6): 369 - 376. [Abstract] [Full Text]