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Radiation-induced Neoplastic Transformation of Human Prostate Epithelial Cells¹

Department of Radiation Medicine, Vincent T. Lombardi Cancer Center, Georgetown University School of Medicine, Washington, DC 20007 [M. R. K., P. J. T., M. J., S. P. V., S. C. P., A. D.], and Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21702 [J. S. R.]

We report the malignant transformation of human prostate epithelial cells (267B1) after multiple exposures to ionizing radiation. Carcinogenic progression of cells from immortal growth to anchorage-independent growth in soft agar to tumorigenicity in athymic mice resulted after a cumulative X-ray dose of 30 Gy. The tumors were characterized histologically as poorly differentiated adenocarcinomas, expressed prostate-specific antigen, and stained positive for keratin. No p53 or ras mutations were observed. Numerous chromosomal defects were noted on karyotypes after radiation exposure. However, chromosome 3 and 8 translocations were observed predominantly in the tumor outgrowths. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to ionizing radiation.

¹ This work was supported in part by National Cancer Institute Grants CA52954 (to P. T.) and R01-CA45408 (to A. D.), both from the United States Department of Health and Human Services, and by the RSNA Research and Education Fund (to M. K.).

² To whom requests for reprints should be addressed, at Department of Radiation Medicine, Vincent T. Lombardi Cancer Center, Georgetown University School of Medicine, Washington, DC 20007. Phone: (202) 784-3328; Fax: (202) 784-3323.

Received 10/20/95. Accepted 11/14/95.

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