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Increase of a Type of Oxidative DNA Damage, 8-Hydroxyguanine, and Its Repair Activity in Human Leukocytes by Cigarette Smoking¹

Department of Environmental Oncology [S. A., T. H., R. Y., H. K.] and First Department of Surgery [H. I.], University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807, and Sumitomo Metal Industries Kokura Steel Works Clinic, I Konomi-cho, Kokurakita-ku, Kitakyushu 802 [Y. T.], Japan

To investigate the oxidative stress induced by cigarette smoking, the levels of a form of oxidative DNA damage, 8-hydroxyguanine (8-OH-Gua), and its repair activity in the leukocytes of current smokers, ex-smokers, and complete nonsmokers were measured. The mean level of 8-OH-Gua was 1.88-fold higher in smokers as compared to complete nonsmokers (the difference was statistically significant, P = 0.013). The mean 8-OH-Gua repair activity was 1.6-fold higher in smokers than complete nonsmokers (the difference was statistically significant, P = 0.0053). A positive association was observed for the 8-OH-Gua levels and its repair activity. Considerable interindividual variations in the 8-OH-Gua levels (current smokers, 3.5-fold; nonsmokers, 7.2-fold) and in its repair activity (current smokers, 7.5-fold; nonsmokers, 5.5-fold) were observed. These results demonstrate that not only smoking status but also life-style, environment, and genetic differences might have effects on the level of 8-OH-Gua and its repair activity in human leukocytes.

¹ This work was supported by a Grant-in-Aid 05270102 for Scientific Research on Priority Areas from The Ministry of Education, Science, Sports and Culture of Japan.

² To whom requests for reprints should be addressed.

Received 11/27/95. Accepted 4/ 3/96.

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