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Hyaluronate-independent Metastatic Behavior of CD44 Variant-expressing Pancreatic Carcinoma Cells¹

Forschungszentrum Karlsruhe, Institute of Genetics, P. O. Box 3640, D-76021 Karlsruhe, Germany [J. P. S., J. F. M., A. H., W. R., L. S. S., H. P., P. H.], and Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020 Salzburg, Austria [S. A., G. K.]

Several studies have demonstrated a correlation between the expression of CD44 variant isoforms and the ability of tumor cells to metastasize. The CD44 proteins carry amino acid sequence motifs that confer the ability to bind to the extracellular matrix component hyaluronate (HA). In this study, we investigated whether a CD44 variant previously shown to stimulate metastasis in a rat pancreatic carcinoma model (BSp73AS) is capable of binding to HA, and whether such binding is critical for metastasis. We show that transfection of this CD44 variant into BSp73AS cells increases the HA-binding capacity of the cells in a dose-dependent manner. Transfection of the same CD44 variant isoform into BDX2 cells also conferred strong HA-binding properties on these cells, but was insufficient to cause them to metastasize. Transfection of a surface-bound hyaluronidase into metastasizing BSp73AS cells bearing variant CD44 efficiently ablated the ability of these cells to bind to HA. However, in metastasis assays, these hyaluronidase-transfected cells showed patterns of metastasis similar to those of the parental cell line. We also show that the HA-binding capacity of a variety of tumor cells is not correlated with their metastatic proclivity, and that an antibody previously shown to block metastasis of the pancreatic carcinoma cells does not interfere with their ability to bind to HA. We conclude that although CD44 variant expression does promote metastasis formation, HA binding by tumor cells is not rate limiting for metastasis in the BSp73AS system and probably also in other metastasizing tumors. Furthermore, for metastasis by CD44 variant-expressing BSp73AS cells to occur, contact of the CD44 variant protein with a ligand other than HA is required.

¹ J. P. S. was supported by a European Molecular Biology Organisation Long-Term Fellowship and by a European Union Human Capital and Mobility Fellowship. The Karlsruhe group was supported by the Deutsche Forschungsgemeinschaft (He 551/8-2) and Boehringer Ingelheim. The Salzburg group was supported by Grant P09888-MOB from the Österreichischer Fonds zur Förderung der Wissenschaftlichen Forschung and the Medizinische Forschungsgemeinschaft.

² To whom requests for reprints should be addressed. Fax: (49) 7247 82 3354.

Received 1/17/96. Accepted 5/ 1/96.

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