| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Divisions of Cell Growth and Regulation [F-L. W., F. J. A., A. B. P.], Cellular and Molecular Biology [Y. W., W-K. W., Y. L., L. B. C.], and Medical Oncology [P. W. K.], Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, and Vanderbilt Cancer Center [P. L.], Nashville, Tennessee 37232
Alterations in transcriptional control may contribute directly to carcinogenesis. Using the differential display technique in prostate cancer cells compared to normal prostate epithelial cells, we identified a down-regulated gene and an up-regulated gene in cancer cells. The down-regulated gene encodes human epithelial tropomyosin (TMe1), a member of the family of actin filament-binding proteins. The up-regulated gene encodes cytochrome c oxidase subunit VIc (COSVIc), a protein of the respiration chain in the mitochondrial inner membrane. The differential display pattern was confirmed by Northern hybridization in both prostate tissue and cell lines. In situ hybridization of malignant prostate epithelial tissue using a digoxigenin-labeled antisense riboprobe detected strong staining for mRNA of COSVIc, as opposed to very weak staining in normal prostate epithelium. The expression pattern of COSVIc may be a useful marker for studying the alteration of energy metabolism in cancer cells and for the diagnosis of prostate cancer.
1 This work was supported by the Dana-Farber Cancer Institute Prostate Cancer Research Foundation.
2 To whom requests for reprints should be addressed, at Division of Cell Growth and Regulation, Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115.
Received 5/16/96. Accepted 7/ 1/96.
This article has been cited by other articles:
|
|
 |
|
  G. Schevzov, B. Vrhovski, N. S. Bryce, S. Elmir, M. R. Qiu, G. M. O'Neill, N. Yang, N. M. Verrills, M. Kavallaris, and P. W. Gunning Tissue-specific Tropomyosin Isoform Composition J. Histochem. Cytochem., May 1, 2005; 53(5): 557 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Shen, M. D. Person, J. Zhu, J. L. Abbruzzese, and D. Li Protein Expression Profiles in Pancreatic Adenocarcinoma Compared with Normal Pancreatic Tissue and Tissue Affected by Pancreatitis as Detected by Two-Dimensional Gel Electrophoresis and Mass Spectrometry Cancer Res., December 15, 2004; 64(24): 9018 - 9026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ruiz, W. Huang, M. E. Hegi, K. Lange, M.-F. Hamou, E. Fluri, E. J. Oakeley, R. Chiquet-Ehrismann, and G. Orend Differential Gene Expression Analysis Reveals Activation of Growth Promoting Signaling Pathways by Tenascin-C Cancer Res., October 15, 2004; 64(20): 7377 - 7385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Bakin, A. Safina, C. Rinehart, C. Daroqui, H. Darbary, and D. M. Helfman A Critical Role of Tropomyosins in TGF-{beta} Regulation of the Actin Cytoskeleton and Cell Motility in Epithelial Cells Mol. Biol. Cell, October 1, 2004; 15(10): 4682 - 4694. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wu, A. Owlia, and P. Singh Precursor peptide progastrin1-80 reduces apoptosis of intestinal epithelial cells and upregulates cytochrome c oxidase Vb levels and synthesis of ATP Am J Physiol Gastrointest Liver Physiol, December 1, 2003; 285(6): G1097 - G1110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Untergasser, H. B. Koch, A. Menssen, and H. Hermeking Characterization of Epithelial Senescence by Serial Analysis of Gene Expression: Identification of Genes Potentially Involved in Prostate Cancer Cancer Res., November 1, 2002; 62(21): 6255 - 6262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. W. Asmann, F. Kosari, K. Wang, J. C. Cheville, and G. Vasmatzis Identification of Differentially Expressed Genes in Normal and Malignant Prostate by Electronic Profiling of Expressed Sequence Tags Cancer Res., June 1, 2002; 62(11): 3308 - 3314. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. B. Deng, H. K. Parekh, K.-C. Chow, and H. Simpkins Increased Expression of Dihydrodiol Dehydrogenase Induces Resistance to Cisplatin in Human Ovarian Carcinoma Cells J. Biol. Chem., April 19, 2002; 277(17): 15035 - 15043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Shields, H. Mehta, K. Pruitt, and C. J. Der Opposing Roles of the Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase Cascades in Ras-Mediated Downregulation of Tropomyosin Mol. Cell. Biol., April 1, 2002; 22(7): 2304 - 2317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Shields, K. Rogers-Graham, and C. J. Der Loss of Transgelin in Breast and Colon Tumors and in RIE-1 Cells by Ras Deregulation of Gene Expression through Raf-independent Pathways J. Biol. Chem., March 15, 2002; 277(12): 9790 - 9799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Iwao, R. Matoba, N. Ueno, A. Ando, Y. Miyoshi, K. Matsubara, S. Noguchi, and K. Kato Molecular classification of primary breast tumors possessing distinct prognostic properties Hum. Mol. Genet., January 1, 2002; 11(2): 199 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-F. Thai, J. W. Allen, A. B. DeAngelo, M. H. George, and J. C. Fuscoe Detection of early gene expression changes by differential display in the livers of mice exposed to dichloroacetic acid Carcinogenesis, August 1, 2001; 22(8): 1317 - 1322. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Waghray, M. Schober, F. Feroze, F. Yao, J. Virgin, and Y. Q. Chen Identification of Differentially Expressed Genes by Serial Analysis of Gene Expression in Human Prostate Cancer Cancer Res., May 1, 2001; 61(10): 4283 - 4286. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. P. Yu, F. Lin, M. Bisceglia, D. Krill, R. Dhir, M. Becich, and J.-H. Luo Identification of a Novel Gene with Increasing Rate of Suppression in High Grade Prostate Cancers Am. J. Pathol., January 1, 2001; 158(1): 19 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nishi, H. Shoji, H. Miyanaka, and T. Nakamura Androgen-Regulated Expression of a Novel Member of the Aldo-Keto Reductase Superfamily in Regrowing Rat Prostate Endocrinology, September 1, 2000; 141(9): 3194 - 3199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tanaka, S. Kondo, Y. Iwasa, H. Hiai, and S. Toyokuni Expression of Stress-Response and Cell Proliferation Genes in Renal Cell Carcinoma Induced by Oxidative Stress Am. J. Pathol., June 1, 2000; 156(6): 2149 - 2157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Gray and C. Collins Genome changes and gene expression in human solid tumors Carcinogenesis, March 1, 2000; 21(3): 443 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Xu, L. Lin, and N. S. Rote Identification of a Stress-Induced Protein During Human Trophoblast Differentiation by Differential Display Analysis Biol Reprod, September 1, 1999; 61(3): 681 - 686. [Abstract] [Full Text]
|
|
|
|
|
|
S. Bao, M.-S. Chang, D. Auclair, Y. Sun, Y. Wang, W.-K. Wong, J. Zhang, Y. Liu, X. Qian, R. Sutherland, et al. HRad17, a Human Homologue of the Schizosaccharomyces pombe Checkpoint Gene rad17, Is Overexpressed in Colon Carcinoma Cancer Res., May 1, 1999; 59(9): 2023 - 2028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Markwalder and J. C. Reubi Gastrin-releasing Peptide Receptors in the Human Prostate: Relation to Neoplastic Transformation Cancer Res., March 1, 1999; 59(5): 1152 - 1159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. J. Janssen, K. G. Veenstra, P. Jonasch, E. Jonasch, and J. W. Mier Ras- and Raf-induced Down-modulation of Non-muscle Tropomyosin Are MEK-independent J. Biol. Chem., November 27, 1998; 273(48): 32182 - 32186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wu, G. N. Rao, B. Dai, and P. Singh Autocrine Gastrins in Colon Cancer Cells Up-regulate Cytochrome c Oxidase Vb and Down-regulate Efflux of Cytochrome c and Activation of Caspase-3 J. Biol. Chem., October 13, 2000; 275(42): 32491 - 32498. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
