This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shimizu, M. Articles by Matsuzawa, A. Search for Related Content PubMed PubMed Citation Articles by Shimizu, M. Articles by Matsuzawa, A.

Augmentation of Antitumor Immunity with Bacterial Superantigen, Staphylococcal Enterotoxin B-bound Tumor Cells

Department of Cancer Therapeutics, Tokyo Metropolitan Institute of Medical Science, Honkomagome 3-18-22, Bunkyo-ku, Tokyo [M. S., A. Y.], and Laboratory Animal Research Center, Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo [H. N., A. K.]

We have examined the effectiveness of a bacterial superantigen, staphylococcal enterotoxin B (SEB)-coupled tumor cells, to induce antitumor activity. SEB was chemically conjugated to tumor cells using a heterobifunctional cross-linking agent acting through NH₂ and SH groups. V ß8⁺ T cells were activated and increased in number after the culture with SEB-bound Meth A cells. The cultured T cells exhibited an antitumor activity in the Winn assay. Interleukin 2 (IL-2) receptor ⁺ (IL-2R⁺) V ß8⁺ T cells but not IL-2R⁺ V ß6⁺ T cells increased in number in mice injected with SEB-bound Meth A cells. However, the percentages of V ß8⁺ and V ß6⁺ T cells did not change by this immunization. The antitumor effector cells were V ß7^-8^- CD4⁺ T cells. In vivo immunization with SEB-bound cells induced a strong antitumor activity, i.e., tumor-free mice/total mice = 14 of 15 (93%) for Meth A and 7 of 15 (47%) for hepatoma MH134. The induced antitumor activity was both dose dependent and tumor specific. Treatment with SEB-bound cells prolonged the survival days of Meth A-bearing mice by 62%. These results suggest that SEB-bound tumor cells may be a powerful method for induction of in vivo antitumor activity.

¹ To whom requests for reprints should be addressed.

Received 8/ 3/95. Accepted 6/17/96.

This article has been cited by other articles:

				J. L. Wahlsten, C. D. Mills, and S. Ramakrishnan Antitumor Response Elicited by a Superantigen- Transmembrane Sequence Fusion Protein Anchored onto Tumor Cells J. Immunol., December 15, 1998; 161(12): 6761 - 6767. [Abstract] [Full Text] [PDF]