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Tamoxifen-induced DNA Adducts in Endometrial Samples from Breast Cancer Patients¹

Department of Biosciences at Novum, Karolinska Institute, Novum, 141 57 Huddinge [K. H., H. R.]; Department of Obstetrics and Gynecology, Oncology-Pathology, University Hospital, 221 85 Lund [B. L.]; and Department of Woman and Child Health, Karolinska Hospital, 171 76 Stockholm [B. M.], Sweden

Tamoxifen-induced DNA adducts were analyzed with the ³²P-postlabeling method using high-performance liquid chromatography (HPLC)-radioactivity detection from endometrial tissue of breast cancer patients and controls. Liver DNA from tamoxifen-treated rats was used as a positive standard. In blind analysis, five of the seven samples from tamoxifen-treated patients showed DNA adducts; none of the five controls were positive. The identity of the tamoxifen adduct was confirmed by using different chromatographic systems, isolating the HPLC fractions and running them on TLC, with or without spiked rat liver samples. The level of adducts in the treated patients was 2.7 adducts/10⁹ nucleotides in the HPLC analysis.

¹ This study was supported by the Swedish Cancer Fund and the Orion-Farmos Company. Presented at the meeting DNA Adducts and Mutations in Human Biomonitoring, Huddinge, Sweden, June 1996.

² To whom requests for reprints should be addressed. Phone: 46-8-6089243; Fax: 46-8-6081501.

Received 7/ 1/96. Accepted 8/15/96.

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