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Loss of Heterozygosity for Chromosome 11 in Adenocarcinoma of the Stomach¹

Jefferson Cancer Institute and Jefferson Cancer Center, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107 [R. B., M. N., B. M., C. M. C.]; Department of Pathology, Padova University, ULSS15 Veneto, Cittadella, 35013 Italy [M. R.]; Department of Genetics and Microbiology, Pavia University, Pavia, 27100 Italy [G. N. R.]; and National Cancer Center Research Institute, Tokyo, 104 Japan [S. H.]

Loss of heterozygosity (LOH) at several chromosomal loci is a common feature of the malignant progression of human tumors. In the case of chromosome 11, LOH has been well documented in several types of solid neoplasms, including gastric carcinoma, suggesting the presence of suppressor gene(s) at 11p15 and 11q22–23. Little is currently known about the molecular events occurring during the development of gastric cancer. To define the regions of chromosome 11 involved in gastric cancer progression, we used high-density polymorphic markers to screen for LOH in matched normal and tumor tissue DNA from 60 primary gastric carcinomas. We found that 21% of the tumors showed LOH simultaneously at 11p15 and 11q22–23, 41% had LOH at 11p15, and 30% had LOH at 11q22–23. We confirm that the minimal critical area of LOH for 11p15.5 is the approximately 2-Mb region between loci D11S1318 and D11S988. However, when we analyzed the pattern of LOH according to the country of origin of the patient, LOH for 11q22–23 alone was found only in cases from Italy. The minimal critical region of LOH at 11q22–23 is identical to that identified for other solid tumors, suggesting that the same putative tumor suppressor gene(s) contained within this region is involved in the pathogenesis of several common human tumors.

¹ This work was supported by National Cancer Institute Outstanding Investigator Award CA39860 (to C. M. C.) and by AIRC (Associazione Italiana per la Ricerca sul Cancro).

² To whom requests for reprints should be addressed, at Thomas Jefferson University, Jefferson Medical College, BLSB, Room 1050, 233 South 10th Street, Philadelphia, PA 19107-5799.

Received 11/16/95. Accepted 12/ 7/95.

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