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Isolation of Prostate-derived Single Cells and Cell Clusters from Human Peripheral Blood¹

Institute of Clinical Chemistry and Laboratory Medicine, Westfälische Wilhelms-Universität, 48149 Münster, Germany [B. B., R. J., C. G., S. H., G. A.]; Institute of Immunology, University Witten/Herdecke, 58448 Witten, Germany [C. G., K. S. Z.]and; Clinic and Policlinic of Urology, Westfälische Wilhelms-Universität, 48149 Münster, Germany [O. B., A. S.]

The detection of blood-borne prostate cancer (PCA) cells may help with clinical staging and the further understanding of PCA metastases. We discovered prostate-specific antigen (PSA)-positive stained but not PSA mRNA-expressing blood cells by means of cell sorting and PSA reverse transcription-PCR in patients. Therefore, we developed a cytokeratin immunomagnetic method to isolate PSA-positive epithelial cells from the circulating blood of PCA patients. We obtained blood-borne single cells from 6 of 10 PCA patients and clustered cells from 8 of 10 PCA patients. Patients with benign prostate hyperplasia tested negative for cell clusters. The reported isolation method yielded prostate-derived cells or clusters of them from PCA-diagnosed patients.

¹ Supported in part by the Fritz-Bender Foundation, Munich.

² To whom requests for reprints should be addressed.

Received 7/ 3/96. Accepted 8/27/96.

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