| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Departments of Surgery [J. P. V., G. H., P. A. F., J. R. M., J. D. I.], Gynecology, Division of Oncology [F. D. C., A. B., P. A. F.], Genetics [P. A. F.], Pathology [J. D. I.], and Cell Biology [J. D. I.], Duke University Medical Center, Durham, North Carolina 27710
Identifying the conditions and kinetics of the induction of BRCA2 gene expression may implicate roles for the function of the tumor suppressor gene. In this study, expression of BRCA2 mRNA is shown to be regulated by the cell cycle and associated with proliferation in normal and tumorderived breast epithelial cells. Cells arrested in G0 or early G1 contained low levels of BRCA2 mRNA. After release into a proliferating state, cells produced maximum levels of BRCA2 mRNA in late G1 and the S-phase. Similar cell cycle control of BRCA2 was observed in fractions of exponentially growing cells isolated by centrifugal elutriation. Expression of BRCA2 was shown to be independent of bulk DNA synthesis. In addition, the kinetics of BRCA2 mRNA up-regulation appeared to be similar to those of BRCA1, suggesting that the two genes could be commonly controlled. These results imply that these two tumor suppressor genes are utilized during growth and may have a protective role in cellular proliferation.
1 Supported by the Duke Breast Cancer Specialized Program of Research Excellence (SPORE) NIH Grant CA68438 and NIH Grant CA63786 (to J. P. V.).
2 To whom requests for reprints should be addressed, at Box 3873, Duke University Medical Center, Durham, NC 27710.
Received 7/16/96. Accepted 8/26/96.
This article has been cited by other articles:
|
|
 |
|
  X. Chen, C. A. Arciero, C. Wang, D. Broccoli, and A. K. Godwin BRCC36 Is Essential for Ionizing Radiation-Induced BRCA1 Phosphorylation and Nuclear Foci Formation. Cancer Res., May 15, 2006; 66(10): 5039 - 5046. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Feng, L. Kachnic, J. Zhang, S. N. Powell, and F. Xia DNA Damage Induces p53-dependent BRCA1 Nuclear Export J. Biol. Chem., July 2, 2004; 279(27): 28574 - 28584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hussain, J. B. Wilson, A. L. Medhurst, J. Hejna, E. Witt, S. Ananth, A. Davies, J.-Y. Masson, R. Moses, S. C. West, et al. Direct interaction of FANCD2 with BRCA2 in DNA damage response pathways Hum. Mol. Genet., June 15, 2004; 13(12): 1241 - 1248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Sharan, A. Pyle, V. Coppola, J. Babus, S. Swaminathan, J. Benedict, D. Swing, B. K. Martin, L. Tessarollo, J. P. Evans, et al. BRCA2 deficiency in mice leads to meiotic impairment and infertility Development, January 1, 2004; 131(1): 131 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-R. Lin, N. S. Y. Ting, J. Qin, and W.-H. Lee M Phase-specific Phosphorylation of BRCA2 by Polo-like Kinase 1 Correlates with the Dissociation of the BRCA2-P/CAF Complex J. Biol. Chem., September 19, 2003; 278(38): 35979 - 35987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wu, S.-W. Jiang, and F. J. Couch p53 Mediates Repression of the BRCA2 Promoter and Down-regulation of BRCA2 mRNA and Protein Levels in Response to DNA Damage J. Biol. Chem., April 25, 2003; 278(18): 15652 - 15660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hilton, J. P. Geisler, J. A. Rathe, M. A. Hattermann-Zogg, B. DeYoung, and R. E. Buller Inactivation of BRCA1 and BRCA2 in Ovarian Cancer J Natl Cancer Inst, September 18, 2002; 94(18): 1396 - 1406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Whitfield, G. Sherlock, A. J. Saldanha, J. I. Murray, C. A. Ball, K. E. Alexander, J. C. Matese, C. M. Perou, M. M. Hurt, P. O. Brown, et al. Identification of Genes Periodically Expressed in the Human Cell Cycle and Their Expression in Tumors Mol. Biol. Cell, June 1, 2002; 13(6): 1977 - 2000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-C. Wang, K. Makino, L.-K. Su, A. Y. Pao, J. Soo Kim, and M.-C. Hung Ultraviolet Irradiation Induces BRCA2 Protein Depletion through a p53-independent and Protein Synthesis-dependent Pathway Cancer Res., April 1, 2001; 61(7): 2838 - 2842. [Abstract] [Full Text]
|
|
|
|
|
|
A.-M. Martin and B. L. Weber Genetic and Hormonal Risk Factors in Breast Cancer J Natl Cancer Inst, July 19, 2000; 92(14): 1126 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Futamura, H. Arakawa, K. Matsuda, T. Katagiri, S. Saji, Y. Miki, and Y. Nakamura Potential Role of BRCA2 in a Mitotic Checkpoint after Phosphorylation by hBUBR1 Cancer Res., March 1, 2000; 60(6): 1531 - 1535. [Abstract] [Full Text]
|
|
|
|
|
|
C.-F. Chen, P.-L. Chen, Q. Zhong, Z. D. Sharp, and W.-H. Lee Expression of BRC Repeats in Breast Cancer Cells Disrupts the BRCA2-Rad51 Complex and Leads to Radiation Hypersensitivity and Loss of G2/M Checkpoint Control J. Biol. Chem., November 12, 1999; 274(46): 32931 - 32935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Cortez, Y. Wang, J. Qin, and S. J. Elledge Requirement of ATM-Dependent Phosphorylation of Brca1 in the DNA Damage Response to Double-Strand Breaks Science, November 5, 1999; 286(5442): 1162 - 1166. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Y. Marmorstein, T. Ouchi, and S. A. Aaronson The BRCA2 gene product functionally interacts with p53 and RAD51 PNAS, November 10, 1998; 95(23): 13869 - 13874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Carney, G. L. Maxwell, J. M. Lancaster, C. Gumbs, J. Marks, A. Berchuck, and P. A. Futreal Aberrant Splicing of the TSG101 Tumor Suppressor Gene in Human Breast and Ovarian Cancers Reproductive Sciences, September 1, 1998; 5(5): 281 - 285. [Abstract] [PDF]
|
|
|
|
|
|
P.-L. Chen, C.-F. Chen, Y. Chen, J. Xiao, Z. D. Sharp, and W.-H. Lee The BRC repeats in BRCA2 are critical for RAD51 binding and resistance to methyl methanesulfonate treatment PNAS, April 28, 1998; 95(9): 5287 - 5292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. C. Wong, R. Pero, P. A. Ormonde, S. V. Tavtigian, and P. L. Bartel RAD51 Interacts with the Evolutionarily Conserved BRC Motifs in the Human Breast Cancer Susceptibility Gene brca2 J. Biol. Chem., December 19, 1997; 272(51): 31941 - 31944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Jin, X. L. Xu, M.-C. W. Yang, F. Wei, T.-C. Ayi, A. M. Bowcock, and R. Baer Cell cycle-dependent colocalization of BARD1 and BRCA1 proteins in discrete nuclear domains PNAS, October 28, 1997; 94(22): 12075 - 12080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Durocher, J. Simard, J. Ouellette, V. Richard, F. Labrie, and G. Pelletier Localization of BRCA1 Gene Expression in Adult Cynomolgus Monkey Tissues J. Histochem. Cytochem., September 1, 1997; 45(9): 1173 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Ludwig, D L Chapman, V E Papaioannou, and A Efstratiadis Targeted mutations of breast cancer susceptibility gene homologs in mice: lethal phenotypes of Brca1, Brca2, Brca1/Brca2, Brca1/p53, and Brca2/p53 nullizygous embryos. Genes & Dev., May 15, 1997; 11(10): 1226 - 1241. [Abstract] [PDF]
|
|
|
|
|
|
A Suzuki, J L de la Pompa, R Hakem, A Elia, R Yoshida, R Mo, H Nishina, T Chuang, A Wakeham, A Itie, et al. Brca2 is required for embryonic cellular proliferation in the mouse. Genes & Dev., May 15, 1997; 11(10): 1242 - 1252. [Abstract] [PDF]
|
|
|
|
|
|
K. Wu, S.-W. Jiang, M. Thangaraju, G. Wu, and F. J. Couch Induction of the BRCA2 Promoter by Nuclear Factor-kappa B J. Biol. Chem., November 3, 2000; 275(45): 35548 - 35556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Sarkisian, S. R. Master, L. J. Huber, S. I. Ha, and L. A. Chodosh Analysis of Murine Brca2 Reveals Conservation of Protein-Protein Interactions but Differences in Nuclear Localization Signals J. Biol. Chem., September 28, 2001; 276(40): 37640 - 37648. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
