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Distinct Patterns of Chromosomal Alterations in High- and Low-Grade Head and Neck Squamous Cell Carcinomas¹

Department of Otorhinolaryngology [U. B.] and Institute of Pathology [A. S., M. D., I. P.], Charité, Humboldt-University Berlin, Schumannstrasse 20-21, D-10098, Berlin, Germany

Comparative genomic hybridization was performed on 30 primary head and neck squamous cell carcinomas. Fractional or entire DNA loss of chromosome 3p was a basic finding that occurred in 29 cases (97%). Additional DNA underrepresentations were observed in more than 50% of the cases on chromosomes 1p, 4, 5q, 6q, 8p, 9p, 11q, 13q, 18q, and 21q. Deletions on chromosomes 3p, 13q, and 17p were confirmed by loss of heterozygosity analysis. Entire or partial DNA copy number increases were identified for chromosome 3q in 26 cases (87%) with high-level amplifications at 3q24 and 3q27-qter. Overrepresentations were found in decreasing order of frequency at 11q13 (70%), 8q (57%), 19q (50%), 19p (47%), and 17q (47%). The use of comparative genomic hybridization superkaryograms of the group of well-differentiated carcinomas (G₁) indicated that the deletions on chromosomes 3p and 9p along with the overrepresentation of 3q are associated with early tumor development. Accordingly, the undifferentiated tumors (G₃) were characterized by additional deletions on chromosomes 4q, 8p, 11q, 13q, 18q, and 21q and overrepresentations on 1pter, 11q13, 19, and 22q, suggesting that these changes are preferentially associated with tumor progression.

¹ Supported by the German Cancer Foundation (DFG, Pe 602/2).

² To whom requests for reprints should be addressed. Phone: 0049-30-2802-2611; Fax: 0049-30-2802-3407.

Received 8/26/96. Accepted 10/17/96.

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