This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kawahara, N. Articles by Kuwano, M. Search for Related Content PubMed PubMed Citation Articles by Kawahara, N. Articles by Kuwano, M.

Enhanced Coexpression of Thioredoxin and High Mobility Group Protein 1 Genes in Human Hepatocellular Carcinoma and the Possible Association with Decreased Sensitivity to Cisplatin¹

Departments of Biochemistry [N. K., A. Y., T. T., M. O., M. W., M. K.] and Second Surgery [K. T., K. S.], Kyushu University School of Medicine, Maidashi, Fukuoka 812-82, and Departments of Health Development [H. N.] and Molecular Biology [K. K.], University of Occupational and Environmental Health, Kitakyushu 807, Japan

Thioredoxin (TRX), a disulfide-reducing intracellular protein, functions as a cellular defense mechanism against oxidative stress. In this study, we asked whether expression of TRX, glutathione-thiol transferase , and high mobility group protein 1 (HMG-1) genes is enhanced in human hepatocellular carcinoma and whether expression of these genes is associated with sensitivity to cisplatin. Both TRX and HMG-1 were co-overexpressed in almost all cancerous lesions in comparison to normal tissue in surgically resected hepatocellular carcinomas of 20 patients. Tumor sensitivity to cisplatin [cis-diamminedichloroplatinum (II)], but not to mitomycin C or doxorubicin, correlated with mRNA levels of TRX in cancer tissue. TRX and HMG-1 may be useful tumor markers, and TRX might be also a useful marker for sensitivity to cisplatin in human hepatocellular carcinomas.

¹ This study was supported by a grant-in-aid for cancer research from the Ministry of Education, Science and Culture, Japan and by grants from the Fukuoka Anticancer Research Fund, Fukuoka 21st Century Medical Fund, and Yasuda Memorial Medical Grant for Cancer Research.

² To whom requests for reprints should be addressed. Phone: 81-92-641-1151, ext. 3354; Fax: 81-92-632-4198.

Received 9/16/96. Accepted 10/16/96.

This article has been cited by other articles:

				R. K. Ramanathan, D. L. Kirkpatrick, C. P. Belani, D. Friedland, S. B. Green, H-H. S. Chow, C. A. Cordova, S. P. Stratton, E. R. Sharlow, A. Baker, et al. A Phase I Pharmacokinetic and Pharmacodynamic Study of PX-12, a Novel Inhibitor of Thioredoxin-1, in Patients with Advanced Solid Tumors Clin. Cancer Res., April 1, 2007; 13(7): 2109 - 2114. [Abstract] [Full Text] [PDF]

				D. Mustacich, A. Wagner, R. Williams, W. Bair, L. Barbercheck, S. P. Stratton, A. K. Bhattacharyya, and G. Powis Increased skin carcinogenesis in a keratinocyte directed thioredoxin-1 transgenic mouse Carcinogenesis, October 1, 2004; 25(10): 1983 - 1989. [Abstract] [Full Text] [PDF]

				N. B. Arnold, K. Ketterer, J. Kleeff, H. Friess, M. W. Buchler, and M. Korc Thioredoxin Is Downstream of Smad7 in a Pathway That Promotes Growth and Suppresses Cisplatin-Induced Apoptosis in Pancreatic Cancer Cancer Res., May 15, 2004; 64(10): 3599 - 3606. [Abstract] [Full Text] [PDF]

				Y. R. Choi, H. Kim, H. J. Kang, N.-G. Kim, J. J. Kim, K.-S. Park, Y.-K. Paik, H. O. Kim, and H. Kim Overexpression of High Mobility Group Box 1 in Gastrointestinal Stromal Tumors with KIT Mutation Cancer Res., May 1, 2003; 63(9): 2188 - 2193. [Abstract] [Full Text] [PDF]

				I. Poser, M. Golob, R. Buettner, and A. K. Bosserhoff Upregulation of HMG1 Leads to Melanoma Inhibitory Activity Expression in Malignant Melanoma Cells and Contributes to Their Malignancy Phenotype Mol. Cell. Biol., April 15, 2003; 23(8): 2991 - 2998. [Abstract] [Full Text] [PDF]

				M. E. Tome, A. F. Baker, G. Powis, C. M. Payne, and M. M. Briehl Catalase-overexpressing Thymocytes Are Resistant to Glucocorticoid-induced Apoptosis and Exhibit Increased Net Tumor Growth Cancer Res., March 1, 2001; 61(6): 2766 - 2773. [Abstract] [Full Text]

				E. Hinoshita, T. Uchiumi, K.-i. Taguchi, N. Kinukawa, M. Tsuneyoshi, Y. Maehara, K. Sugimachi, and M. Kuwano Increased Expression of an ATP-binding Cassette Superfamily Transporter, Multidrug Resistance Protein 2, in Human Colorectal Carcinomas Clin. Cancer Res., June 1, 2000; 6(6): 2401 - 2407. [Abstract] [Full Text]

				A. J. Freemerman, A. Gallegos, and G. Powis Nuclear Factor {{kappa}}B Transactivation Is Increased But Is Not Involved in the Proliferative Effects of Thioredoxin Overexpression in MCF-7 Breast Cancer Cells Cancer Res., August 1, 1999; 59(16): 4090 - 4094. [Abstract] [Full Text] [PDF]