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Elevation of Interferon ß-inducible Proteins in Ataxia Telangiectasia Cells¹

School of Kinesiology, Simon Fraser University, Burnaby, British Columbia, V5A 1S6 Canada [C. S. A., M. P. R.], and Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226 [A. L. H.]

The recently cloned ATM gene has been shown to bear considerable homology to phosphatidylinositol 3 kinases and, therefore, its product may function in signal transduction. In this study, we report constitutively elevated levels of two IFN-ß-inducible proteins, ubiquitin cross-reactive protein (UCRP), and low molecular weight protein (LMP2), in human fibroblasts with the inherited disease ataxia telangiectasia (AT). Using immunoblotting, it was found that a M_r 15,000 band representing free UCRP was hardly detectable in normal cells, while it was the predominant band in AT cells. Similarly, the expression of a M_r 23,000 protein, LMP2, was found to be higher in AT cells than in normal cells. Culturing three successive passages of the AT cell line in the presence of different concentrations of neutralizing antibodies against IFN-ß caused partial and complete reduction, respectively, of the free UCRP and LMP2 signals to normal levels. These results indicate that UCRP and LMP2 pools may be basally elevated in AT cells due to constitutive activation of the IFN-ß induction pathway and are in keeping with the recently reported constitutive activation of the NF-B transcriptional activator in AT cells.

¹ This investigation was supported by a grant from the Natural Sciences and Engineering Council of Canada.

² To whom requests for reprints should be addressed, at School of Kinesiology, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.

Received 11/21/95. Accepted 12/14/95.

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