This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reid, L. H. Articles by Weissman, B. E. Search for Related Content PubMed PubMed Citation Articles by Reid, L. H. Articles by Weissman, B. E.

Genomic Organization of the Human p57^KIP2 Gene and Its Analysis in the G401 Wilms' Tumor Assay¹

Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, North Carolina 27599 [L. H. R., S. J. C-M., A. W., B. E. W.], and Cell Biology and Genetics Program, Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, New York, New York 10021 [M-H. L. J. M.]

The p57^KIP2 gene encodes an inhibitor of cyclin-dependent kinase activity, which negatively regulates cell cycle progression. The human p57 gene is located in 11p15.5, a region of DNA frequently altered in neoplasia. We have isolated a human genomic clone and mapped the p57 gene to a 2.2-kb region between D11S648 and D11S679. Sequence analysis revealed that the coding DNA of the human p57 gene is divided by a 0.5-kb intron. A second intron was detected in the 3' untranslated region, indicating that the human p57 gene contains at least three exons. Our previous work with somatic cell hybrids mapped a tumor suppressor gene for the G401 Wilms' tumor cell line to a 500-kb region of 11p15.5 that includes p57. Northern blot analysis detected a 0.8-kb p57 transcript in several of the G401 hybrid lines. However, p57 expression did not correlate with tumor suppression. These results suggest that p57 is not responsible for the tumor suppression observed in our somatic cell hybrid assay.

¹ This research was supported by NIH Grant CA63176 (to B. E. W.) and by the Howard Hughes Medical Institute. L. H. R. was an American Cancer Society Postdoctoral Fellow. S. J. C-M. is an NIH Predoctoral Fellow supported by Environmental Pathology Training Grant ES07017.

² To whom requests for reprints should be addressed, at Department of Pathology and Laboratory Medicine, 345 Lineberger Cancer Center, CB#7295, University of North Carolina, Chapel Hill, NC 27599.

Received 12/11/95. Accepted 1/31/96.

This article has been cited by other articles:

				R. J. Jin, Y. Lho, Y. Wang, M. Ao, M. P. Revelo, S. W. Hayward, M. L. Wills, S. K. Logan, P. Zhang, and R. J. Matusik Down-regulation of p57Kip2 Induces Prostate Cancer in the Mouse Cancer Res., May 15, 2008; 68(10): 3601 - 3608. [Abstract] [Full Text] [PDF]

				S. Engemann, M. Strodicke, M. Paulsen, O. Franck, R. Reinhardt, N. Lane, W. Reik, and J. Walter Sequence and functional comparison in the Beckwith-Wiedemann region: implications for a novel imprinting centre and extended imprinting Hum. Mol. Genet., November 1, 2000; 9(18): 2691 - 2706. [Abstract] [Full Text] [PDF]

				A. Tsugu, K. Sakai, P. B. Dirks, S. Jung, R. Weksberg, Y.-L. Fei, S. Mondal, S. Ivanchuk, C. Ackerley, P. A. Hamel, et al. Expression of p57KIP2 Potently Blocks the Growth of Human Astrocytomas and Induces Cell Senescence Am. J. Pathol., September 1, 2000; 157(3): 919 - 932. [Abstract] [Full Text] [PDF]

				S. Lai, H. Goepfert, A. M. Gillenwater, M. A. Luna, and A. K. El-Naggar Loss of Imprinting and Genetic Alterations of the Cyclin-dependent Kinase Inhibitor p57KIP2 Gene in Head and Neck Squamous Cell Carcinoma Clin. Cancer Res., August 1, 2000; 6(8): 3172 - 3176. [Abstract] [Full Text]

				J. Liu, A. I. Kahri, P. Heikkila, and R. Voutilainen Ribonucleic Acid Expression of the Clustered Imprinted Genes, p57KIP2, Insulin-Like Growth Factor II, and H19, in Adrenal Tumors and Cultured Adrenal Cells J. Clin. Endocrinol. Metab., June 1, 1997; 82(6): 1766 - 1771. [Abstract] [Full Text] [PDF]