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6ß1-Integrin, a Major Cell Surface Carrier of ß1-6-branched Oligosaccharides, Mediates Migration of EJ-ras-transformed Fibroblasts on Laminin-1 Independently of Its Glycosylation State¹

Ludwig Institute for Cancer Research, Sao Paulo, SP [M. G. J., R. C., A. M. V., R. R. B.], and Disciplina de Biologia Celular, Escola Paulista de Medicina, Universidade Federal de Sao Paulo, Sao Paulo, SP [L. R. T.], Brasil

EJ-ras oncogene-induced malignant transformation is characterized by a series of changes in cell surface carbohydrates and cell-cell and cell-matrix interactions. Here, we show that EJ-ras-transformed NIH-3T3 fibroblasts acquired a migratory phenotype on laminin-1 surfaces. Such a phenotype was accompanied by overexpression of: (a) functional 6ß1, but not other laminin binding ß1-integrins; and (b) glycoconjugates on the cell surface bearing large oligosaccharides recognized by leukoagglutinin from Phaseolus vulgaris (L-PHA). The internal pool of pre-ß1-integrins was differently regulated in EJ-ras-transformed cells compared with nontransfected fibroblasts. Conversion of pre-ß1- into mature ß1-integrins was faster in EJ-ras-transformed cells, a process associated with the overexpression of the 6-chain. Overexpression of L-PHA-reactive oligosaccharides is dependent on the activity of N-acetylglucosaminyltransferase V, which is increased in transformed cells [J. W. Dennis et al., Science (Washington DC), 236: 582–585, 1987]. We show that ß1-integrins were the major carriers of L-PHA-reactive oligosaccharides on the cell surface. This glycosylation pattern, however, was not necessary for either the cell surface expression of ß1-integrins or their functional activity in the migratory response to laminin-1. Moreover, EJ-ras-transformed fibroblasts aggregated spontaneously. These effects were not observed in c-jun-transfected fibroblasts, which were unable to migrate on laminin, did not overexpress either ß1-integrins or L-PHA-reactive oligosaccharides, and did not self-aggregate.

¹ Supported by CNPq Brasil Grants 840178/95-6 (M. G. J.) and 200015/94-0 (R. C.).

² To whom requests for reprints should be addressed, at Glycobiology Program, Cancer Center, 0687, University of California San Diego School of Medicine, La Jolla, CA 92093-0687. Phone: (619) 534-1346; Fax: (619) 534-5611; E-mail: rchammas@ucsd.edu.

³ Present address: Departamento de Microbiologia. Instituto de Ciencias Biomédicas, Universidade de Sao Paulo. Sao Paulo. SP. Brasil.

Received 9/27/95. Accepted 2/ 1/96.

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