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Absence of G_s Gene Mutations in Childhood Thyroid Tumors afte Chernobyl in Contrast to Sporadic Adult Thyroid Neoplasia¹

Institute of Pathology, Ludwig Maximilians University of Munich, Thalkirchner Strasse 36, D-80337 München, Germany

Heterotrimeric G proteins participate in the signal transduction cascade. Adult thyroid tumors have been shown to harbor specific point mutations in codons 201 and 227 of the G_s subunit of the adenylate cyclase stimulator. This protein affects the GDP/GTP turnover and finally results in an enhanced activation of G_s and thus adenylate cyclase. We attempted to find out if G_s gene mutations were present in thyroid tumors of children from Belarus after the Chernobyl nuclear accident. Paraffin sections of 20 thyroid tumors were used for PCR amplification by oligonucleotide intron primers flanking exons 8 and 9, encompassing codon 201 and 227, respectively. By direct sequencing of the 274-bp amplification product, we did not detect any mutations of the G_s gene in codon 201 or 227. In contrast to thyroid neoplasia of adults, G_s gene mutations do not play a role in the development of childhood thyroid tumors after the Chernobyl reactor accident.

¹ This work was supported by grants from Dr. Mildred Scheel-Stiftung für Krebsforschung, Bonn, Germany, and Wilhelm Sander Stiftung, Neuburg an der Donau, Germany.

² To whom requests for reprints should be addressed. Phone: (49) 89-5160-4081; Fax: (49) 89-5160-4083.

Received 3/ 4/97. Accepted 4/30/97.

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