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Differential Display Cloning Identifies Motility-related Protein (MRP1/CD9) As Highly Expressed in Primary Compared to Metastatic Human Colon Carcinoma Cells¹

Department of Experimental Pathology, Institut Suisse de Recherches Experimentales sur le Cancer, 155 Chemin des Boveresses, 1066 Epalinges, Switzerland

Differential display cloning was performed to analyze genes that are differentially expressed in matched primary and metastases-derived human colon carcinoma cell lines. This led to the identification of PMA16, a gene identical to the previously cloned motility-related protein gene (MRP1/CD9). Northern and Western blot analyses of cell lines, as well as immunostaining of tissue sections from the original tumor surgical samples, confirmed that MRP1/CD9 was highly expressed at the primary site, compared to the low levels of expression in metastases. We also demonstrated that primary colon cancer cells displayed a significantly higher migration potential, compared to metastasis-derived cells. Antibodies directed against MRP1/CD9 largely prevented cell migration in vitro, but they did not influence cell adhesion. Thus, differential display cloning has allowed for the identification of MRP1/CD9, a motility-related gene product, which may regulate the metastatic phenotype of human colon cancer.

¹ This work was supported by the Swiss National Science Foundation (Grants 31-33993.92 and 31-043500.95), Cancer Research Switzerland (Grants AKT 589 and 277-1-1996), a fellowship from Institut Suisse de Recherches Experimentales sur le Cancer (to T. S.), and a grant from the Charles Veillon Foundation.

² To whom requests for reprints should be addressed.

Received 2/17/97. Accepted 5/15/97.

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