| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Institut National de la Santé et de la Recherche Médicale CJF 94-08. Faculté de Medecine, 7 Boulevard Jeanne d'Arc, 21033 Dijon Cedex, France [C. G., P. O., A. F., A. H., B. C.], and Laboratoire du Stress Cellulaire, Centre National de la Recherche Scientifique UMR-5534, Université Claude Bernard Lyon-1, 69622 Villeurbanne, France [A-P. A., P. M.]
Resistance of colorectal cancer cells to chemotherapeutic drugs increases as cells reach confluence. Here we show that the small stress protein HSP27, which has been described to block necrotic and apoptotic cell death, accumulates in confluent human colorectal cancer cell lines HT-29 and Caco2. Cell confluence also induces HSP27 phosphorylation and changes in its intracellular distribution. We also show that overexpression of human HSP27 by transfection of HT-29 cells increased the resistance of cells to doxorubicin or cisplatin and prevented drug-induced apoptosis. Interestingly, nonconfluent HSP27-transfected cells and confluent control cells in which HSP27 is expressed at the same level displayed a similar drug resistance. HSP27-transfected cells did not exhibit an enhanced resistance when they reached confluence, nor was there an increased accumulation of HSP27. We have previously shown that HSP27 expression blocks tumor necrosis factor-induced cell death as a result of decreasing intracellular reactive oxygen species (ROS). Here we show that HSP27 overexpression in HT-29 cells, obtained either by transfection or by growing the cells at high density, correlated with a significant ROS decrease. We conclude that cell confluent-dependent HSP27 accumulation, probably due to its ability to decrease ROS levels, is essential for the establishment of the resistance of colorectal cancer cells when reaching confluence.
1 Supported by the Conseil Regional de la Bourgogne, the Ligue Bourguignonne contre le Cancer, Association pour la Recherche sur le Cancer Grant 6011, Institut National pour la Santé et la Recherche Medicale Grant 930.501, and the Region Rhône-Alpes.
2 To whom requests for reprints should be addressed. Phone: 33-03-80-67-1788.
Received 12/10/96. Accepted 4/30/97.
This article has been cited by other articles:
|
|
 |
|
  A. Havasi, Z. Li, Z. Wang, J. L. Martin, V. Botla, K. Ruchalski, J. H. Schwartz, and S. C. Borkan Hsp27 Inhibits Bax Activation and Apoptosis via a Phosphatidylinositol 3-Kinase-dependent Mechanism J. Biol. Chem., May 2, 2008; 283(18): 12305 - 12313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Huang, J. B. Fileta, T. Filippopoulos, A. Ray, A. Dobberfuhl, and C. L. Grosskreutz Hsp27 Phosphorylation in Experimental Glaucoma Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 4129 - 4135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wu, H. Kausar, P. Johnson, D. E. Montoya-Durango, M. Merchant, and M. J. Rane Hsp27 Regulates Akt Activation and Polymorphonuclear Leukocyte Apoptosis by Scaffolding MK2 to Akt Signal Complex J. Biol. Chem., July 27, 2007; 282(30): 21598 - 21608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhang and X. Shen Heat Shock Protein 27 Protects L929 Cells from Cisplatin-Induced Apoptosis by Enhancing Akt Activation and Abating Suppression of Thioredoxin Reductase Activity Clin. Cancer Res., May 15, 2007; 13(10): 2855 - 2864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Alford, S. Glennie, B. R. Turrell, L. Rawlinson, J. Saklatvala, and J. L. E. Dean Heat Shock Protein 27 Functions in Inflammatory Gene Expression and Transforming Growth Factor-beta-activated Kinase-1 (TAK1)-mediated Signaling J. Biol. Chem., March 2, 2007; 282(9): 6232 - 6241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Schmitt, M. Gehrmann, M. Brunet, G. Multhoff, and C. Garrido Intracellular and extracellular functions of heat shock proteins: repercussions in cancer therapy J. Leukoc. Biol., January 1, 2007; 81(1): 15 - 27. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Parcellier, M. Brunet, E. Schmitt, E. Col, C. Didelot, A. Hammann, K. Nakayama, K. I. Nakayama, S. Khochbin, E. Solary, et al. HSP27 favors ubiquitination and proteasomal degradation of p27Kip1 and helps S-phase re-entry in stressed cells FASEB J, June 1, 2006; 20(8): 1179 - 1181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Schmitt, L. Maingret, P.-E. Puig, A.-L. Rerole, F. Ghiringhelli, A. Hammann, E. Solary, G. Kroemer, and C. Garrido Heat shock protein 70 neutralization exerts potent antitumor effects in animal models of colon cancer and melanoma. Cancer Res., April 15, 2006; 66(8): 4191 - 4197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zuidervaart, P. J. Hensbergen, M.-C. Wong, A. M. Deelder, C. P. Tensen, M. J. Jager, and N. A. Gruis Proteomic analysis of uveal melanoma reveals novel potential markers involved in tumor progression. Invest. Ophthalmol. Vis. Sci., March 1, 2006; 47(3): 786 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rocchi, E. Beraldi, S. Ettinger, L. Fazli, R. L. Vessella, C. Nelson, and M. Gleave Increased Hsp27 after Androgen Ablation Facilitates Androgen-Independent Progression in Prostate Cancer via Signal Transducers and Activators of Transcription 3-Mediated Suppression of Apoptosis Cancer Res., December 1, 2005; 65(23): 11083 - 11093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-J. Lee, D.-H. Lee, C.-K. Cho, S. Bae, G.-J. Jhon, S.-J. Lee, J.-W. Soh, and Y.-S. Lee HSP25 Inhibits Protein Kinase C{delta}-mediated Cell Death through Direct Interaction J. Biol. Chem., May 6, 2005; 280(18): 18108 - 18119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Whitlock, K. Lindsey, N. Agarwal, C. E. Crosson, and J.-X. Ma Heat Shock Protein 27 Delays Ca2+-Induced Cell Death in a Caspase-Dependent and -Independent Manner in Rat Retinal Ganglion Cells Invest. Ophthalmol. Vis. Sci., March 1, 2005; 46(3): 1085 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Theriault, H. Lambert, A. T. Chavez-Zobel, G. Charest, P. Lavigne, and J. Landry Essential Role of the NH2-terminal WD/EPF Motif in the Phosphorylation-activated Protective Function of Mammalian Hsp27 J. Biol. Chem., May 28, 2004; 279(22): 23463 - 23471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chauhan, G. Li, T. Hideshima, K. Podar, C. Mitsiades, N. Mitsiades, L. Catley, Y. T. Tai, T. Hayashi, R. Shringarpure, et al. Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance Blood, November 1, 2003; 102(9): 3379 - 3386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-J. Park, R. B. Gaynor, and Y. T. Kwak Heat Shock Protein 27 Association with the I{kappa}B Kinase Complex Regulates Tumor Necrosis Factor {alpha}-induced NF-{kappa}B Activation J. Biol. Chem., September 12, 2003; 278(37): 35272 - 35278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Parcellier, E. Schmitt, S. Gurbuxani, D. Seigneurin-Berny, A. Pance, A. Chantome, S. Plenchette, S. Khochbin, E. Solary, and C. Garrido HSP27 Is a Ubiquitin-Binding Protein Involved in I-{kappa}B{alpha} Proteasomal Degradation Mol. Cell. Biol., August 15, 2003; 23(16): 5790 - 5802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sakamoto, T. Mashima, K. Yamamoto, and T. Tsuruo Modulation of Heat-shock Protein 27 (Hsp27) Anti-apoptotic Activity by Methylglyoxal Modification J. Biol. Chem., November 22, 2002; 277(48): 45770 - 45775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Paul, F. Manero, S. Gonin, C. Kretz-Remy, S. Virot, and A.-P. Arrigo Hsp27 as a Negative Regulator of Cytochrome c Release Mol. Cell. Biol., February 1, 2002; 22(3): 816 - 834. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Takeno, T. Noguchi, R. Kikuchi, T. Sato, Y. Uchida, and S. Yokoyama Analysis of the Survival Period in Resectable Stage IV Gastric Cancer Ann. Surg. Oncol., April 1, 2001; 8(3): 215 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Cornford, A. R. Dodson, K. F. Parsons, A. D. Desmond, A. Woolfenden, M. Fordham, J. P. Neoptolemos, Y. Ke, and C. S. Foster Heat Shock Protein Expression Independently Predicts Clinical Outcome in Prostate Cancer Cancer Res., December 1, 2000; 60(24): 7099 - 7105. [Abstract] [Full Text]
|
|
|
|
|
|
S. J. Charette, J. N. Lavoie, H. Lambert, and J. Landry Inhibition of Daxx-Mediated Apoptosis by Heat Shock Protein 27 Mol. Cell. Biol., October 15, 2000; 20(20): 7602 - 7612. [Abstract] [Full Text]
|
|
|
|
|
|
S. Dorion, J. Berube, J. Huot, and J. Landry A Short Lived Protein Involved in the Heat Shock Sensing Mechanism Responsible for Stress-activated Protein Kinase 2 (SAPK2/p38) Activation J. Biol. Chem., December 31, 1999; 274(53): 37591 - 37597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. GARRIDO, J.-M. BRUEY, A. FROMENTIN, A. HAMMANN, A. P. ARRIGO, and E. SOLARY HSP27 inhibits cytochrome c-dependent activation of procaspase-9 FASEB J, November 1, 1999; 13(14): 2061 - 2070. [Abstract] [Full Text]
|
|
|
|
|
|
V. Chaturvedi, J.-Z. Qin, M. F. Denning, D. Choubey, M. O. Diaz, and B. J. Nickoloff Apoptosis in Proliferating, Senescent, and Immortalized Keratinocytes J. Biol. Chem., August 13, 1999; 274(33): 23358 - 23367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Lambert, S. J. Charette, A. F. Bernier, A. Guimond, and J. Landry HSP27 Multimerization Mediated by Phosphorylation-sensitive Intermolecular Interactions at the Amino Terminus J. Biol. Chem., April 2, 1999; 274(14): 9378 - 9385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Piotrowicz, E. Hickey, and E. G. Levin Heat shock protein 27 kDa expression and phosphorylation regulates endothelial cell migration FASEB J, November 1, 1998; 12(14): 1481 - 1490. [Abstract] [Full Text]
|
|
|
|
|
|
K. A. Buzzard, A. J. Giaccia, M. Killender, and R. L. Anderson Heat Shock Protein 72 Modulates Pathways of Stress-induced Apoptosis J. Biol. Chem., July 3, 1998; 273(27): 17147 - 17153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Katschinski, K. Boos, S. G. Schindler, and J. Fandrey Pivotal Role of Reactive Oxygen Species as Intracellular Mediators of Hyperthermia-induced Apoptosis J. Biol. Chem., July 7, 2000; 275(28): 21094 - 21098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. I. Bello, C. Gomez-Diaz, F. Navarro, F. J. Alcain, and J. M. Villalba Expression of NAD(P)H:Quinone Oxidoreductase 1 in HeLa Cells. ROLE OF HYDROGEN PEROXIDE AND GROWTH PHASE J. Biol. Chem., November 21, 2001; 276(48): 44379 - 44384. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Cell Growth & Differentiation |
