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Departments of Obstetrics and Gynecology [H. T., Y. Y., J. C., T. H. P., G. P. P., T. J. O.], Pathology [S. K.], and Biochemistry and Molecular Biology [T. J. O.], University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205-7199, and Department of Obstetrics and Gynecology, Hiroshima University School of Medicine, Hiroshima, Japan 737 [K. S.]
Extracellular proteases mediate the digestion of neighboring extracellular matrix components in initial tumor growth, allow shedding or desquamation of tumor cells into the surrounding environment, provide the basis for invasion of basement membranes in target metastatic organs, and are required for release and activation of many growth and angiogenic factors. We identified overexpression of the serine protease hepsin gene in ovarian carcinomas and investigated the expression of this gene in 44 ovarian tumors (12 low malignant potential tumors and 32 carcinomas) and 10 normal ovaries. Quantitative PCR was used to determine the relative expression of hepsin compared to that of ß-tubulin. The mRNA expression levels of hepsin were significantly elevated in 7 of 12 low malignant potential tumors and in 27 of 32 carcinomas. On Northern blot analysis, the hepsin transcript was abundant in carcinoma but was almost never expressed in normal adult tissue, including normal ovary. Our results suggest that hepsin is frequently overexpressed in ovarian tumors and therefore may be a candidate protease in the invasive process and growth capacity of ovarian tumor cells.
1 To whom requests for reprints should be addressed, at Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, 4301 West Markham Slot #718. Little Rock. AR 72205-7199.
Received 5/ 8/97. Accepted 5/29/97.
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