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Departments of Medicine [M. P. L., A. P. F.], Molecular Biology & Genetics, and Oncology [A. P. F.]. The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
The 11p15 gene TSG101 was recently reported to undergo frequent large intragenic deletions in human breast cancer. Here we show that that is generally not the case, but the gene shows aberrant splicing, based on the following observations: identical products were observed in matching normal and fetal tissues; deleted cDNA sequence revealed canonical splicing donor and acceptor site sequences; and genomic Southern blots showed no intragenic deletions in all 72 tumors studied. Nevertheless, relaxation of RNA splicing fidelity may be an oncodevelopmental marker in cancer and may play a general role in other genes and tumors.
1 This work was supported by Grant DAMD17-94-J-4308.
2 To whom requests for reprints should be addressed, at The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Ross 1064, Baltimore, MD 21205.
Received 5/13/97. Accepted 6/12/97.
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