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Inhibition of Cytochromes P-450 and Induction of Glutathione S-Transferases by Sulforaphane in Primary Human and Rat Hepatocytes¹

INSERM U456, Détoxication et Réparation Tissulaire, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, 35043 Rennes cedex, France [K. M., F. M., S. L., E. L. F., A. G.], and Department of Oncology, University College Medical School, London W1P 8BT, United Kingdom [H. K., B. K.]

The isothiocyanate sulforaphane (SF) is thought to be a potential chemoprotective agent. Its effects on Phase I and Phase II enzymes of carcinogen metabolism in primary cultures of rat and human hepatocytes have been investigated. Northern blot analyses of rat hepatocytes showed a dose-dependent induction of mRNAs for rat glutathione S-transferases (rGSTs) A1/A2 and P1 but not M1. This was associated with enhanced levels of not only rGSTA1, A2, A4, A5, and P1 but also of rGSTs M1 and M2. On the other hand, the enzyme activities in rat hepatocytes associated with cytochromes P-450 (CYPs) 1A1 and 2B1/2, namely ethoxyresorufin-O-deethylase and pentoxyresorufin-O-dealkylase, respectively, were decreased in a dose-dependent manner. In SF-treated human hepatocytes, hGSTA1/2 but not hGSTM1 mRNAs were induced, and the expression of CYP1A2 was unaffected, whereas the expression of CYP3A4, the major CYP in human liver, was markedly decreased at both mRNA and activity levels. These observations demonstrate that in intact human and rat hepatocytes, SF may both induce a number of GSTs and cause enzyme inhibition of some but not all CYPs and, in the case of CYP3A4, inhibit both its enzyme activity and its expression.

¹ Supported by the Institut National de la Santé et de la Recherche Médicale, the Association pour la Recherche sur le Cancer, and the European Communities (BIOMED, Contract BMH4-CT96-0254). K. M. is a recipient of a fellowship from the Ministère de la Recherche et de l'Enseignement Supérieur. S. L. is a recipient of a fellowship from the Association pour la Recherche sur le Cancer.

² To whom requests for reprints should be addressed, at INSERM U456, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Rennes 1, 2 avenue Léon Bernard, 35043 Rennes cedex, France. Phone: 33-2-99-33-62-41; Fax: 33-2-99-33-62-42; E-mail: Fabrice.Morel@univ-rennesl.fr.

Received 6/10/97. Accepted 7/14/97.

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