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Growth of Human Nondiploid Primary Prostate Tumor Epithelial Cells in Vitro¹

Institute of Chemical Toxicology, Karmanos Cancer Institute [D. P. C., A. W.], and the Department of Pathology, Wayne State University [F. H. S., D. J. G., W. A. S., A. M.], Detroit, Michigan 48201

Research into molecular and cellular defects underlying prostate cancer would be advanced by in vitro models of prostate tumor cells representing patient tumors. We have propagated, in serum-free medium, epithelial cell cultures derived from nondiploid prostate tumors and normal human prostate. The serial passage tumor cells exhibited nondiploid karyotype and transformed phenotypes of focus formation and anchorage-independent growth. In contrast, the normal prostate cells showed diploid karyotype and lacked transformed phenotypes. Both the tumor and normal cells were positive for prostate-specific antigen and cytokeratins 18 and 19 and negative for keratin 15. These results demonstrate that the nondiploid prostate tumors and normal prostate epithelial cell cultures retained their respective in vivo properties and should allow studies to elucidate molecular alterations involved in human prostate cancer.

¹ This work was supported by USPHS Development Grant RC21CA69845 and the Electron Microscopy in Situ Hybridization Core Facility of the National Institute of Environmental Health Sciences Center Grant P30ES06639.

² To whom requests for reprints should be addressed, at the Institute of Chemical Toxicology, 2727 Second Avenue, Room 4000, Detroit, Michigan 48201. Phone: (313) 577-0093; Fax: (313) 577-0082.

Received 6/ 9/97. Accepted 7/15/97.

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