This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morelli, C. Articles by Barbanti-Brodano, G. Search for Related Content PubMed PubMed Citation Articles by Morelli, C. Articles by Barbanti-Brodano, G.

Characterization of a 4-Mb Region at Chromosome 6q21 Harboring a Replicative Senescence Gene¹

Institute of Microbiology and Interdepartment Center for Biotechnology, School of Medicine, University of Ferrara, I-44100 Ferrara, Italy [C. M., C. T., P. R., F. G., M. N., G. B-B.], and Department of Cancer Genetics, Paterson Institute for Cancer Research, Manchester M20 9BX, United Kingdom [T. S., M. J. G., J. M. B.]

A 4-Mb region containing a senescence gene was defined at 6q21 by fluorescence in situ hybridization and deletion mapping after transfer of a normal human chromosome 6 to a BK virus-transformed mouse cell line. By screening three different yeast artificial chromosome (YAC) libraries, a YAC contig was constructed that covers the deleted region at 6q21. The contig is composed of 18 overlapping YACs with a size of 250–1800 kb and contains 3 CpG islands and 10 expressed sequence tags. By sequencing YACs and P1 artificial chromosomes, nine new sequence tagged sites and three new expressed sequence tags were detected that enrich the genetic resources of the region. The contig may also contain a fragile site, FRA6F, located close to a CpG island, which could be a landmark to localize the senescence gene. This YAC contig will be used to detect expressed sequences to clone and characterize the senescence gene at 6q21.

¹ Supported by grants from Associazione Italiana per la Ricerca sul Cancro (to G. B-B. and M. N.), Consiglio Nazionale delle Ricerche Progetto Finalizzato "Applicazioni Cliniche della Ricerca Oncologicala" and Ministero dell'Università e Ricerca Scientifica e Tecnologica 60% (to G. B-B.), and the United Kingdom Cancer Research Campaign and European Commission BioMed 1 Contract GENE-CT93-0075 (to J. M. B.). C. M. was supported by a fellowship from Fondazione Italiana per la Ricerca sul Cancro.

² To whom requests for reprints should be addressed, at the Institute of Microbiology, University of Ferrara, Via Luigi Borsari, 46, I-44100 Ferrara, Italy. Phone: 39-522-291406; Fax: 39-532-247618; E-mail: bev@dns.unife.it.

Received 5/27/97. Accepted 8/15/97.

This article has been cited by other articles:

				Q. M. CHEN Replicative Senescence and Oxidant-Induced Premature Senescence: Beyond the Control of Cell Cycle Checkpoints Ann. N.Y. Acad. Sci., June 1, 2000; 908(1): 111 - 125. [Abstract] [Full Text] [PDF]