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Pretreatment Prediction of the Chemotherapeutic Response of Human Glioma Cell Cultures Using Nuclear Magnetic Resonance Spectroscopy and Artificial Neural Networks¹

Department of Neurological Surgery, Institute of Neurology, London, WC1N 3BG [W. E-D., S. M. A., N. M. B., J. L. D., D. G. T. T.], and The Royal College of Surgeons Unit of Biophysics, Institute of Child Health, London, WC1N 1EH [S. R. W.], United Kingdom

Both tumor metabolism and its response to cytotoxic drugs are intrinsic properties of tumor cells. It is therefore likely that there is a relationship between the two properties, however subtle and complex, wherein the metabolic characteristics of tumor cells can reflect the inherent response (resistance or sensitivity) of these cells to cytotoxic drugs. We used artificial neural network analysis to show that it is possible to distinguish, prior to treatment, between drug-resistant and drug-sensitive human glioma cell cultures from their metabolic profiles, as given by high-resolution proton nuclear magnetic resonance spectra of the cell extracts, and to predict their cellular response to the chemotherapeutic drug 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea in vitro. The results suggest that neural network analysis of tumor nuclear magnetic resonance spectra has potential as a prognostic tool for determining treatment of gliomas, ultimately noninvasively, and may be used to provide information about the metabolic pathways involved in drug response that may be helpful in developing novel treatments for these tumors.

¹ Supported by the Cancer Research Campaign.

² To whom requests for reprints should be addressed, at Department of Neurological Surgery, Institute of Neurology, Queen Square, London, WC1N 3BG, United Kingdom. Fax: 44-171-278-7894; E-mail: W.ElDeredy@ion.ucl.ac.uk.

Received 1/28/97. Accepted 8/18/97.