This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhong, Q. Articles by Lee, W.-H. Search for Related Content PubMed PubMed Citation Articles by Zhong, Q. Articles by Lee, W.-H.

Identification of Cellular TSG101 Protein in Multiple Human Breast Cancer Cell Lines¹

Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center, San Antonio, Texas 78245

tsg101 was identified as a tumor susceptibility gene by homozygous functional inactivation of allelic loci in mouse 3T3 fibroblasts. The human homologue was mapped at chromosome 11p15.1–2 and found to have intragenic deletion in 7 of 15 breast cancer specimens. To further confirm the relevance of defects in this gene to breast cancer, antibodies specific for the putative gene product were prepared and used to identify cellular TSG101 protein. The antibodies recognized a 46-kDa protein in human retinoblastoma WERI-27 cells labeled with [³⁵S]methionine. This protein was not detected with preimmune sera. In cell fractionation studies, the 46-kDa protein cofractionating with glutathione S-transferase was found mainly in the cytoplasm. Similarly, when cells were immunostained with anti-TSG101 antibodies, fluorescence was localized in the cytoplasm of most of the cells. A full-size 46-kDa TSG101 protein was detected in a panel of 10 breast cancer cell lines and 2 normal breast epithelial cell lines with the same antibodies. Consistently, the full-length TSG101 mRNA was also detected in these breast cells using reverse transcription-PCR. These results indicate that homozygous intragenic deletion of TSG101 is rare in breast cancer cells.

¹ Supported in part by NIH Grants CA58318 and P50-CA58183 and by the Alice McDermott Endowment Fund. Q. Z. and C-F. C. contributed equally to this work.

² To whom requests for reprints should be addressed, at Department of Molecular Medicine and Institute of Biotechnology, University of Texas Health Science Center, 15355 Lambda Drive, San Antonio, TX 78245. Phone: (210) 567-7353; Fax: (210) 567-7377.

Received 7/15/97. Accepted 8/15/97.

This article has been cited by other articles:

				T. W. Young, D. G. Rosen, F. C. Mei, N. Li, J. Liu, X.-F. Wang, and X. Cheng Up-regulation of Tumor Susceptibility Gene 101 Conveys Poor Prognosis through Suppression of p21 Expression in Ovarian Cancer Clin. Cancer Res., July 1, 2007; 13(13): 3848 - 3854. [Abstract] [Full Text] [PDF]

				A. Kikonyogo, F. Bouamr, M. L. Vana, Y. Xiang, A. Aiyar, C. Carter, and J. Leis Proteins related to the Nedd4 family of ubiquitin protein ligases interact with the L domain of Rous sarcoma virus and are required for gag budding from cells PNAS, September 13, 2001; (2001) 201268998. [Abstract] [Full Text] [PDF]

				G. H. Feng, C.-J. Lih, and S. N. Cohen TSG101 Protein Steady-State Level Is Regulated Posttranslationally by an Evolutionarily Conserved COOH-Terminal Sequence Cancer Res., March 1, 2000; 60(6): 1736 - 1741. [Abstract] [Full Text]

				Q. Zhong, C. Chen, S. Li, Y. Chen, C. Wang, J. Xiao, P. Chen, Z. D. Sharp, and W. Lee Association of BRCA1 with the hRad50-hMre11-p95 Complex and the DNA Damage Response Science, July 30, 1999; 285(5428): 747 - 750. [Abstract] [Full Text]

				Y. Li, T. Kane, C. Tipper, P. Spatrick, and D. D. Jenness Yeast Mutants Affecting Possible Quality Control of Plasma Membrane Proteins Mol. Cell. Biol., May 1, 1999; 19(5): 3588 - 3599. [Abstract] [Full Text] [PDF]

				W. Xie, L. Li, and S. N. Cohen Cell cycle-dependent subcellular localization of the TSG101 protein and mitotic and nuclear abnormalities associated with TSG101 deficiency PNAS, February 17, 1998; 95(4): 1595 - 1600. [Abstract] [Full Text] [PDF]

				A. Kikonyogo, F. Bouamr, M. L. Vana, Y. Xiang, A. Aiyar, C. Carter, and J. Leis Proteins related to the Nedd4 family of ubiquitin protein ligases interact with the L domain of Rous sarcoma virus and are required for gag budding from cells PNAS, September 25, 2001; 98(20): 11199 - 11204. [Abstract] [Full Text] [PDF]