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Expression of Three UDP-N-acetyl--D-galactosamine:Polypeptide GalNAc N-Acetylagalactosaminyltransferases in Adenocarcinoma Cell Lines¹

Eppley Institute for Cancer and Allied Disease [M. E. S., T. C., T. I., M. A. H.] and Division of Pediatric Gastroenterology, Department of Pediatrics [D. M.], University of Nebraska Medical Center, Omaha, Nebraska 68198-6805; First Department of Medicine, Kochi Medical School, Kochi, Japan [I. N.]; Faculty of Health Sciences, School of Dentistry, Copenhagen, Denmark [E. P. B., H. H., U. M., H. C.]; and First Department of Surgery, Miyazaki Medical College, Miyazaki, Japan [T. I.]

The levels of mRNA expression of three UDP-N-acetyl--D-galactosamine:polypeptide GalNAc N-acetylgalactosaminyltransferases (GalNAc-transferases) were quantified for human adenocarcinoma cell lines from pancreas, colon, stomach, and breast. Two of the GalNAc-transferases, GalNAc-T1 and GalNAc-T2, were expressed constitutively and at low levels in most or all cell lines examined. A third GalNAc-transferase, GalNAc-T3, was differentially expressed. Well-differentiated adenocarcinoma cell lines expressed high levels and moderately differentiated cell lines expressed lower levels of GalNAc-T3. Cell lines classified as poorly differentiated failed to express GalNAc-T3 mRNA at levels that could be detected by Northern blot analysis. Differential expression of the GalNAc-T3 protein was confirmed in these cell lines by Western blotting. We propose that glycosylation in tumor cell lines may be regulated in part by differential expression of GalNAc-transferases, and we suggest that GalNAc-T3 gene expression may be a molecular indicator of differentiated adenocarcinoma.

¹ This work was supported by Grants CA69234, DK46589, and CA36727 from the NIH; grants from The Danish Cancer Society, The Roikjer Foundation, and The Danish Research Council; and Grant-in-Aid for International Scientific Research 09044318 from the Japanese Ministry of Education, Science, Sports and Culture.

² Present address: H. W. Manter Laboratory of Parasitology, University of Nebraska State Museum, University of Nebraska-Lincoln, Lincoln, NE 68588-0514.

³ To whom requests for reprints should be addressed, at Eppley Institute for Cancer and Allied Disease, University of Nebraska Medical Center, 600 South 42nd Street, Omaha, NE 68198-6805. Phone: (402) 559-8343; Fax: (402) 559-4651.

Received 6/13/97. Accepted 9/ 5/97.

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