This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosin, M. P. Articles by Zhang, L. Search for Related Content PubMed PubMed Citation Articles by Rosin, M. P. Articles by Zhang, L.

The Use of Exfoliative Cell Samples to Map Clonal Genetic Alterations in the Oral Epithelium of High-Risk Patients¹

British Columbia Cancer Research Centre, Vancouver, British Columbia, V5Z 4E6 Canada [M. P. R., J. B. E., J. H., T. Z.]; Vancouver General Hospital, Vancouver, British Columbia, V5Z 1M9 Canada [J. B. E., K. B., S. D.]; School of Kinesiology, Simon Fraser University, Burnaby, British Columbia, V5A 1S6 Canada [M. P. R., X. C., T. Z., Y. H.]; and Faculty of Dentistry, the University of British Columbia, Vancouver, British Columbia, V6T 1Z3 Canada [L. Z.]

Although it is widely accepted that clonal genetic alterations are an essential component of tumor progression, little is known of the distribution of such changes in high-risk lesions or how such clones are altered over time. We explored the feasibility of using exfoliative cells collected by scraping the mucosal surface to detect allelic loss in oral lesions of 22 patients (14 squamous cell carcinomas, 2 carcinomas in situ, and 6 dysplasias). The data show that the patterns of allelic loss observed in these samples closely represent those observed in biopsies of the same region. Furthermore, early indications are that this approach can be used to detect recurrent outgrowth of clones of altered cells in patients after therapy.

¹ Supported by grants from the William and Ada Isabelle Steel Fund at Simon Fraser University and by the National Cancer Institute of Canada with funds from the Canadian Cancer Society.

² To whom requests for reprints should be addressed, at Faculty of Dentistry, the University of British Columbia, 2199 Wesbrook Mall, Vancouver, British Columbia, V6T 1Z3 Canada. Phone: (604) 822-6337; Fax: (604) 822-8279; E-mail: lzhang@unixg.ubc.ca.

Received 8/25/97. Accepted 10/17/97.

This article has been cited by other articles:

				Y. Li, M. A. R. St. John, X. Zhou, Y. Kim, U. Sinha, R. C. K. Jordan, D. Eisele, E. Abemayor, D. Elashoff, N.-H. Park, et al. Salivary Transcriptome Diagnostics for Oral Cancer Detection Clin. Cancer Res., December 15, 2004; 10(24): 8442 - 8450. [Abstract] [Full Text] [PDF]

				B. J. M. Braakhuis, C. R. Leemans, R. H. Brakenhoff, M. P. Rosin, W. L. Lam, and L. Zhang Correspondence re: M. P. Rosin et al., 3p14 and 9p21 Loss Is a Simple Tool for Predicting Second Oral Malignancy at Previously Treated Oral Cancer Sites. Cancer Res., 62: 6447-6450, 2002. Cancer Res., August 15, 2003; 63(16): 5167 - 5169. [Full Text] [PDF]

				M. P. Rosin, W. L. Lam, C. Poh, N. D. Le, R. J. Li, T. Zeng, R. Priddy, and L. Zhang 3p14 and 9p21 Loss Is a Simple Tool for Predicting Second Oral Malignancy at Previously Treated Oral Cancer Sites Cancer Res., November 15, 2002; 62(22): 6447 - 6450. [Abstract] [Full Text] [PDF]

				S. M. Lippman and W. K. Hong Cancer Prevention Science and Practice Cancer Res., September 15, 2002; 62(18): 5119 - 5125. [Full Text] [PDF]

				A. K. El-Naggar, L. Mao, G. Staerkel, M. M. Coombes, S. L. Tucker, M. A. Luna, G. L. Clayman, S. Lippman, and H. Goepfert Genetic Heterogeneity in Saliva from Patients with Oral Squamous Carcinomas: Implications in Molecular Diagnosis and Screening J. Mol. Diagn., November 1, 2001; 3(4): 164 - 170. [Abstract] [Full Text] [PDF]

				M. F. Spafford, W. M. Koch, A. L. Reed, J. A. Califano, L. H. Xu, C. F. Eisenberger, L. Yip, P. L. Leong, L. Wu, S. X. Liu, et al. Detection of Head and Neck Squamous Cell Carcinoma among Exfoliated Oral Mucosal Cells by Microsatellite Analysis Clin. Cancer Res., March 1, 2001; 7(3): 607 - 612. [Abstract] [Full Text]

				J. J. Lee, W. K. Hong, W. N. Hittelman, L. Mao, R. Lotan, D. M. Shin, S. E. Benner, X.-C. Xu, J. S. Lee, V. M. Papadimitrakopoulou, et al. Predicting Cancer Development in Oral Leukoplakia: Ten Years of Translational Research Clin. Cancer Res., May 1, 2000; 6(5): 1702 - 1710. [Abstract] [Full Text]

				J. Califano, W. H. Westra, G. Meininger, R. Corio, W. M. Koch, and D. Sidransky Genetic Progression and Clonal Relationship of Recurrent Premalignant Head and Neck Lesions Clin. Cancer Res., February 1, 2000; 6(2): 347 - 352. [Abstract] [Full Text]

				M. P. Rosin, X. Cheng, C. Poh, W. L. Lam, Y. Huang, J. Lovas, K. Berean, J. B. Epstein, R. Priddy, N. D. Le, et al. Use of Allelic Loss to Predict Malignant Risk for Low-grade Oral Epithelial Dysplasia Clin. Cancer Res., February 1, 2000; 6(2): 357 - 362. [Abstract] [Full Text]