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Overexpression of Phospholipase C-1 in Rat 3Y1 Fibroblast Cells Leads to Malignant Transformation¹

Department of Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk 790-784 [M. J. K., H. S., S. H. R., P-G. S.]; Department of Biology, Daejin University, Pochongun, Kyunggido 487-800 [J-S. C.]; Department of Surgery, College of Medicine, Seoul National University, Seoul 110-744 [D. Y. N.]; and Department of Pathology, College of Medicine, Seoul National University, Seoul 110-744 [I. A. P.], South Korea

Phospholipase C-1 (PLC-1) mediates signals from various extracellular origins to evoke cellular events such as mitogenesis. Previously, we reported that PLC-1 was highly expressed in colorectal cancer and familial adenomatous polyposis, suggesting that PLC-1 might be oncogenic. In this study, we have established rat 3Y1 fibroblasts that overexpress whole PLC-1 and src homology 2 (SH2)-SH2-SH3 domain of PLC-1. These cells showed a transformed phenotype and were tumorigenic when transplanted into nude mice. These results indicate that overexpression of PLC-1 could transform rat fibroblasts, and the transformation is mediated by SH2-SH2-SH3 domain of PLC-1.

¹ This investigation was supported by Grant KOSEF 961-0100-001-2 from Korea Science and Engineering Foundation and '97 Good Health R & D Project, The Ministry of Health & Welfare, Republic of Korea.

² To whom requests for reprints should be addressed, at Department of Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk 791-784, South Korea. Phone: 82-562-279-2293; Fax: 82-562-279-2199; E-mail: pgs@vision.postech.ac.kr.

Received 9/ 3/97. Accepted 10/27/97.

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