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Nuclear Location and Cell Cycle Regulation of the BRCA2 Protein¹

Cancer Research Campaign Center for Cell and Molecular Biology [D. B., S. S., N. J. M., C. J. M., A. A.] and Section of Gene Function and Regulation [A. A.], Chester Beatty Laboratories, The Institute of Cancer Research, London SW3 6JB, United Kingdom, and Section of Cell Biology and Experimental Pathology, Haddow Laboratories, The Institute of Cancer Research, Belmont, Sutton, Surrey SM2 5NG, United Kingdom [L. E. J., S. C., M. R. C.]

Women carrying a germ-line mutation in the BRCA1 or BRCA2 genes have a high risk of developing breast cancer, and loss of the wild-type allele in tumors suggests that these genes function as tumor suppressor genes. The BRCA2 gene encodes a 3418-amino acid protein with no significant sequence similarity to any known protein. To begin to elucidate the cellular role of BRCA2, we have raised antibodies to the BRCA2 protein and used these to study its subcellular localization and expression. We show that BRCA2 is a nuclear protein expressed in response to cell proliferation and that BRCA2 expression is initiated before DNA synthesis.

¹ Supported by the Cancer Research Campaign.

² To whom requests for reprints should be addressed, at CRC Centre for Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, Fulham Road, London SW3 6JB, United Kingdom, Phone: 0171-352-8133; Fax: 0171-352-3299; E-mail: alana@icr.ac.uk.

Received 9/26/97. Accepted 10/29/97.

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