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Expression of Sex Hormone-binding Globulin Exon VII Splicing Variant Messenger RNA in Human Uterine Endometrial Cancers

Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu 500, Japan

We have demonstrated the intracellular expression of sex hormone-binding globulin (SHBG) exon VII splicing variant mRNA in human uterine endometrial cancer using the reverse transcription-PCR-Southern blot and DNA sequencing analyses. Analysis of the missing base pairs proved that they corresponded to the entire exon VII, which is considered to encode a portion of the steroid-binding site, suggesting that the steroid-binding affinity of this variant might be different from that of the SHBG wild type. In uterine endometrial cancers, the wild-type mRNA levels significantly (P < 0.01) decreased, and the ratio of the SHBG variant to wild-type mRNA levels (P < 0.01) increased with the advance of histological dedifferentiation. These results suggest that dedifferentiation of endometrial cancers might induce a reduction in their estrogen-dependent properties via intracellular SHBG.

¹ To whom requests for reprints should be addressed, at Department of Obstetrics and Gynecology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500, Japan. Phone: 81 58-267-2631; Fax: 81 58-265-9006.

Received 5/13/97. Accepted 10/15/97.

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