This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bertin, N. Articles by Frappart, L. Search for Related Content PubMed PubMed Citation Articles by Bertin, N. Articles by Frappart, L.

Thrombospondin-1 and -2 Messenger RNA Expression in Normal, Benign, and Neoplastic Human Breast Tissues: Correlation with Prognostic Factors, Tumor Angiogenesis, and Fibroblastic Desmoplasia¹

Department of Pathology and CNRS UMR 5641 [N. B., L. F.] and INSERM Research Unit 403, Pavillon F [P. C.], Edouard Herriot Hospital, Place d'Arsonval, 69437 Lyon Cédex 03, France, and Department of Oncology, Medical Academy of Lodz, UL Kosciuszki 4, Lodz, Poland [R. K.]

Thrombospondin-1 (TSP1) is a M_r 450,000 extracellular matrix glycoprotein that modulates tumor growth, angiogenesis, and metastasis. Of the five structurally different TSPs described to date, only TSP2 is similar to TSP1 in terms of its molecular architecture, and TSP2 also modulates angiogenesis. Angiogenesis plays a relevant role in the biological aggressiveness of breast cancer, and TSP1 is present in the tumor stroma (termed desmoplasia) of invasive human breast ductal carcinoma not otherwise specified (NOS). The present study was designed to identify and quantify TSP1 and TSP2 mRNAs in normal, benign, and neoplastic human breast tissues using the reverse transcriptase PCR technique. We found that TSP2, like TSP1, was expressed in human breast tissues, and that TSP1 and TSP2 mRNA expression in invasive breast carcinoma NOS was significantly increased compared to that observed in normal and benign tissues. The expression of TSP1 and TSP2 in invasive breast ductal carcinoma NOS did not significantly correlate with any of the prognostic factors studied (tumor size, lymph node status, morphology, and hormone receptor status). However, when our study population was divided according to the quantity of tumor stroma, TSP1 (and possibly TSP2) mRNA expression and microvessel counts in desmoplastic-rich stroma of breast carcinoma NOS were significantly increased compared to those observed in desmoplastic-poor stromata.

¹ This work was performed in collaboration with the Institut de Biologie et Chimie des Protéines (Lyon, France) and was supported by grants from the Association pour la Recherche contre le Cancer (to N. B. and L. F.), the Ligue contre le Cancer de la Drome et de l'Ardèche (to N. B), the Fédération Nationale des Groupements des Entreprises Françaises dans la Lutte contre le Cancer (to P. C.), and the Institut National de Santé et de Recherche Médicale (to P. C.).

² To whom requests for reprints should be addressed.

Received 10/11/96. Accepted 12/18/96.

This article has been cited by other articles:

				M.-P. Wu, M.-J. Young, C.-C. Tzeng, C.-R. Tzeng, K.-F. Huang, L.-W. Wu, and C.-Y. Chou A novel role of thrombospondin-1 in cervical carcinogenesis: inhibit stroma reaction by inhibiting activated fibroblasts from invading cancer Carcinogenesis, June 1, 2008; 29(6): 1115 - 1123. [Abstract] [Full Text] [PDF]

				A. Manzo, S. Bugatti, R. Caporali, R. Prevo, D. G. Jackson, M. Uguccioni, C. D. Buckley, C. Montecucco, and C. Pitzalis CCL21 Expression Pattern of Human Secondary Lymphoid Organ Stroma Is Conserved in Inflammatory Lesions with Lymphoid Neogenesis Am. J. Pathol., November 1, 2007; 171(5): 1549 - 1562. [Abstract] [Full Text] [PDF]

				C. D. Sutton, K. O'Byrne, J. C. Goddard, L.-J. Marshall, L. Jones, G. Garcea, A. R. Dennison, G. Poston, D. M. Lloyd, and D. P. Berry Expression of Thrombospondin-1 in Resected Colorectal Liver Metastases Predicts Poor Prognosis Clin. Cancer Res., September 15, 2005; 11(18): 6567 - 6573. [Abstract] [Full Text] [PDF]

				T. A. Rege, C. Y. Fears, and C. L. Gladson Endogenous inhibitors of angiogenesis in malignant gliomas: Nature's antiangiogenic therapy Neuro-oncol, April 1, 2005; 7(2): 106 - 121. [Abstract] [PDF]

				M. J. Calzada, L. Zhou, J. M. Sipes, J. Zhang, H. C. Krutzsch, M. L. Iruela-Arispe, D. S. Annis, D. F. Mosher, and D. D. Roberts {alpha}4{beta}1 Integrin Mediates Selective Endothelial Cell Responses to Thrombospondins 1 and 2 In Vitro and Modulates Angiogenesis In Vivo Circ. Res., March 5, 2004; 94(4): 462 - 470. [Abstract] [Full Text] [PDF]

				P. P. Manna and W. A. Frazier CD47 Mediates Killing of Breast Tumor Cells via Gi-Dependent Inhibition of Protein Kinase A Cancer Res., February 1, 2004; 64(3): 1026 - 1036. [Abstract] [Full Text] [PDF]

				L. S. Gutierrez, M. Suckow, J. Lawler, V. A. Ploplis, and F. J. Castellino Thrombospondin 1--a regulator of adenoma growth and carcinoma progression in the APCMin/+ mouse model Carcinogenesis, February 1, 2003; 24(2): 199 - 207. [Abstract] [Full Text] [PDF]

				A J Rice, M A Steward, and C M Quinn Thrombospondin 1 protein expression relates to good prognostic indices in ductal carcinoma in situ of the breast J. Clin. Pathol., December 1, 2002; 55(12): 921 - 925. [Abstract] [Full Text] [PDF]

				A Rice and C M Quinn Angiogenesis, thrombospondin, and ductal carcinoma in situ of the breast J. Clin. Pathol., August 1, 2002; 55(8): 569 - 574. [Abstract] [Full Text] [PDF]

				G. Page, S. Lebecque, and P. Miossec Anatomic Localization of Immature and Mature Dendritic Cells in an Ectopic Lymphoid Organ: Correlation with Selective Chemokine Expression in Rheumatoid Synovium J. Immunol., May 15, 2002; 168(10): 5333 - 5341. [Abstract] [Full Text] [PDF]

				S.-i. Aishima, K.-i. Taguchi, K. Sugimachi, Y. Asayama, H. Nishi, M. Shimada, K. Sugimachi, and M. Tsuneyoshi The Role of Thymidine Phosphorylase and Thrombospondin-1 in Angiogenesis and Progression of Intrahepatic Cholangiocarcinoma International Journal of Surgical Pathology, January 1, 2002; 10(1): 47 - 56. [Abstract] [PDF]

				J. Lawler, W.-M. Miao, M. Duquette, N. Bouck, R. T. Bronson, and R. O. Hynes Thrombospondin-1 Gene Expression Affects Survival and Tumor Spectrum of p53-Deficient Mice Am. J. Pathol., November 1, 2001; 159(5): 1949 - 1956. [Abstract] [Full Text] [PDF]

				J. Kodama, I. Hashimoto, N. Seki, A. Hongo, M. Yoshinouchi, H. Okuda, and T. Kudo Thrombospondin-1 and -2 Messenger RNA Expression in Invasive Cervical Cancer: Correlation with Angiogenesis and Prognosis Clin. Cancer Res., September 1, 2001; 7(9): 2826 - 2831. [Abstract] [Full Text] [PDF]

				S. Filleur, O. V. Volpert, A. Degeorges, C. Voland, F. Reiher, P. Clezardin, N. Bouck, and F. Cabon In vivo mechanisms by which tumors producing thrombospondin 1 bypass its inhibitory effects Genes & Dev., June 1, 2001; 15(11): 1373 - 1382. [Abstract] [Full Text] [PDF]

				F. de Fraipont, M. El Atifi, C. Gicquel, X. Bertagna, E. M. Chambaz, and J. J. Feige Expression of the Angiogenesis Markers Vascular Endothelial Growth Factor-A, Thrombospondin-1, and Platelet-Derived Endothelial Cell Growth Factor in Human Sporadic Adrenocortical Tumors: Correlation with Genotypic Alterations J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4734 - 4741. [Abstract] [Full Text]

				M. Streit, L. Riccardi, P. Velasco, L. F. Brown, T. Hawighorst, P. Bornstein, and M. Detmar Thrombospondin-2: A potent endogenous inhibitor of tumor growth and angiogenesis PNAS, December 21, 1999; 96(26): 14888 - 14893. [Abstract] [Full Text] [PDF]

				J. Cinatl Jr., R. Kotchetkov, M. Scholz, J. Cinatl, J.-U. Vogel, P. H. Driever, and H. W. Doerr Human Cytomegalovirus Infection Decreases Expression of Thrombospondin-1 Independent of the Tumor Suppressor Protein p53 Am. J. Pathol., July 1, 1999; 155(1): 285 - 292. [Abstract] [Full Text] [PDF]

				M. Nishizaki, T. Fujiwara, T. Tanida, A. Hizuta, H. Nishimori, T. Tokino, Y. Nakamura, M. Bouvet, J. A. Roth, and N. Tanaka Recombinant Adenovirus Expressing Wild-Type p53 Is Antiangiogenic: A Proposed Mechanism for Bystander Effect Clin. Cancer Res., May 1, 1999; 5(5): 1015 - 1023. [Abstract] [Full Text] [PDF]

				L. F. Brown, A. J. Guidi, S. J. Schnitt, L. Van De Water, M. L. Iruela-Arispe, T.-K. Yeo, K. Tognazzi, and H. F. Dvorak Vascular Stroma Formation in Carcinoma in Situ, Invasive Carcinoma, and Metastatic Carcinoma of the Breast Clin. Cancer Res., May 1, 1999; 5(5): 1041 - 1056. [Abstract] [Full Text] [PDF]

				G. Fontanini, L. Boldrini, A. Calcinai, S. Chine, M. Lucchi, A. Mussi, C. A. Angeletti, F. Basolo, and G. Bevilacqua Thrombospondins I and II Messenger RNA Expression in Lung Carcinoma: Relationship with p53 Alterations, Angiogenic Growth Factors, and Vascular Density Clin. Cancer Res., January 1, 1999; 5(1): 155 - 161. [Abstract] [Full Text] [PDF]