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Ludwig Institute for Cancer Research [J. F. C., W. A., H-J. S. H., W. K. C.], Department of Medicine [H-J. S. H., W. K. C.], and Center for Molecular Genetics [W. K. C.], University of California-San Diego, La Jolla, California 92093-0660; Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York, 14263 [C. P., V. M. C., W. A. H.]; Cancer Biology Program, Stanford University, Stanford, California 94305 [W. A.]; and Department of Neurological Surgery, University of Washington School of Medicine, Seattle, Washington 89195 [M. S. B.]
DNA amplification is a common mechanism invoked by many human tumors to elicit overexpression of genes whose products are involved in drug resistance or cell proliferation. Although amplified regions in tumor DNA may exceed several megabases in size, segments of amplicons with a high probability of containing gene sequences may be amenable to detection by restriction landmark genomic scanning (RLGS), a high-resolution DNA analysis that separates labeled NotI fragments in two dimensions. Here, we tested this by applying RLGS to matched samples of glioma and normal brain DNA and found tumor-specific amplification of the gene encoding cyclin-dependent kinase 6 (CDK6), an observation not previously reported in human tumors. The CDK6 gene has been localized to chromosome 7q21–22, but in the gliomas studied here, it was not coamplified with either the syntenic MET (7q31) or epidermal growth factor receptor (7p11-p12) genes, suggesting that this may be part of a novel amplicon in gliomas. We then corroborated this finding by identifying both amplification-associated and amplification-independent increases in CDK6 protein levels in gliomas relative to matched normal brain samples. These data implicate the CDK6 gene in genomic amplification and illustrate the potential of RLGS for the more general identification and cloning of novel genes that are amplified in human cancer.
1 J. F. C. was supported in part by NIH Grant 5T32CA09290.
2 To whom requests for reprints should be addressed, at Ludwig Institute for Cancer Research, 9500 Gilman Drive, La Jolla, CA 92093-0660. Fax: (619) 534-7816; E-mail: jfcostello@ucsd.edu.
Received 12/11/96. Accepted 2/14/97.
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