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Overexpression of Cyclin D1 in Human Pancreatic Carcinoma Is Associated with Poor Prognosis¹

Department of General Surgery, University of Ulm, Steinhövelstrasse 9, 89075 Ulm, Germany

We have investigated the expression of cyclin D1 in adenocarcinoma of the pancreas and the relevance of cyclin D1 expression to clinical outcome. In comparison to normal pancreas, Southern blot analyses revealed amplification of the cyclin D1 coding gene in 25% of the cases, whereas with reverse transcription-PCR, overexpression of mRNA was observed in 82% of the examined tissues. Immunohistochemically, we could demonstrate nuclear overexpression in tumor cells in 68.4%, and this protein accumulation correlated significantly with poor prognosis [median survival, 18.1 versus 10.5 months; P < 0.01 (x² test)].

¹ This work was supported by Grant GA 541/1-1 (to S. G.) from the Deutsche Forschungsgemeinschaft.

² To whom requests for reprints should be addressed. Phone: (49) 0731-502-7200. Fax: (49) 0731-502-7209.

Received 12/30/96. Accepted 3/20/97.

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