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[Cancer Research 58, 23-28, January 1, 1998]
© 1998 American Association for Cancer Research
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Precise Microdissection of Human Prostate Cancers Reveals Genotypic Heterogeneity1

Catherine A. Macintosh, Michael Stower, Nick Reid and Norman J. Maitland2

Yorkshire Cancer Research Campaign Cancer Research Unit, Department of Biology, University of York, York, YO1 5YW [C. A. M., N. J. M.]; Department of Urology, York District Hospital, York, YO3 7HE [M. S.]; and Department of Histopathology, York District Hospital, Wigginton Road, York, YO3 7HE [N. R.] United Kingdom

To determine the incidence of genetic heterogeneity in primary prostate cancer, we have microdissected 125 tumor and mesenchymal foci from 18 patient biopsies and analyzed the DNA for loss of heterozygosity using PCR microsatellite markers. In 100% of patients with genetic lesions on chromosome 8p, there was evidence for intra-tumoral genetic heterogeneity. There was also a low but significant incidence of loss of heterozygosity in mesenchymal tissue. Our results show that phenotypically similar tumor foci can have different genotypes and provide evidence for the multifocality of tumor development in the prostate.

1 This work was supported by the Yorkshire Cancer Research Campaign.

2 To whom requests for reprints should be addressed, at Cancer Research Unit, Department of Biology, University of York, P. O. Box 373, York Y01 5YW, United Kingdom.

Received 5/29/97. Accepted 11/11/97.




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HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
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Copyright © 1998 by the American Association for Cancer Research.