| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Departments of Obstetrics and Gynecology/Division of Gynecologic Oncology [G. L. M., A. B., P. A. F.], Surgery [C. G., P. A. F.], Medicine [H. S.], and Pathology [R. C. B.], Duke University Medical Center, Durham, North Carolina 27710, and Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709 [J. I. R., J. C. B.]
Mutation and deletion of the PTEN tumor suppressor gene occurs in about 40% of endometrial carcinomas. The purpose of this study was to determine whether PTEN mutations also are present in endometrial hyperplasias, which are premalignant precursors of invasive endometrial adenocarcinomas. Genomic DNA from 51 endometrial hyperplasias was extracted from paraffin blocks, and PCR was used to amplify the nine exons of the PTEN gene. These products were screened using single-strand conformation analysis, and variant bands were sequenced. Somatic mutations in the PTEN gene were seen in 10 of 51 cases (20%), and two mutations were found in one case. An identical 4-bp deletion in exon 8 was seen in three cases, and 8 of 11 PTEN mutations predicted truncated protein products. There was no higher frequency of PTEN mutations in endometrial hyperplasias with atypia (6 of 32; 19%) relative to those without atypia (4 of 19; 21%). These data suggest that inactivation of the PTEN tumor suppressor gene is an early event in the development of some endometrial cancers.
1 Supported in part by the Duke University Specialized Programs of Research Excellence in Breast Cancer from the National Cancer Institute.
2 To whom requests for reprints should be addressed, at Division of Gynecologic Oncology, Duke University Medical Center, Box 3079, Durham, NC 27710. Phone: (919) 684-3765; Fax: (919) 684-8719.
Received 3/ 5/98. Accepted 4/28/98.
This article has been cited by other articles:
|
|
 |
|
  G. W. Montgomery, D. R. Nyholt, Z. Z. Zhao, S. A. Treloar, J. N. Painter, S. A. Missmer, S. H. Kennedy, and K. T. Zondervan The search for genes contributing to endometriosis risk Hum. Reprod. Update, September 1, 2008; 14(5): 447 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Treloar, Z. Z. Zhao, L. Le, K. T. Zondervan, N. G. Martin, S. Kennedy, D. R. Nyholt, and G. W. Montgomery Variants in EMX2 and PTEN do not contribute to risk of endometriosis Mol. Hum. Reprod., August 1, 2007; 13(8): 587 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M Haughian, T. A Jackson, D. M Koterwas, and A. P Bradford Endometrial cancer cell survival and apoptosis is regulated by protein kinase C {alpha} and {delta} Endocr. Relat. Cancer, December 1, 2006; 13(4): 1251 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Hayes, H. Wang, R. Espinal-Witter, W. Douglas, G. J. Solomon, S. J. Baker, and L. H. Ellenson PIK3CA and PTEN Mutations in Uterine Endometrioid Carcinoma and Complex Atypical Hyperplasia. Clin. Cancer Res., October 15, 2006; 12(20): 5932 - 5935. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Hecht and G. L. Mutter Molecular and Pathologic Aspects of Endometrial Carcinogenesis J. Clin. Oncol., October 10, 2006; 24(29): 4783 - 4791. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Sansal and W. R. Sellers The Biology and Clinical Relevance of the PTEN Tumor Suppressor Pathway J. Clin. Oncol., July 15, 2004; 22(14): 2954 - 2963. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-S. Liu, J.-T. Dong, J.-T. Chen, Y.-T. Hsieh, E. S.-C. Ho, M.-J. Hung, C.-H. Lu, and L.-C. Chiou KAI1 Metastasis Suppressor Protein Is Down-Regulated during the Progression of Human Endometrial Cancer Clin. Cancer Res., April 1, 2003; 9(4): 1393 - 1398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Risinger, G. L. Maxwell, G. V. R. Chandramouli, A. Jazaeri, O. Aprelikova, T. Patterson, A. Berchuck, and J. C. Barrett Microarray Analysis Reveals Distinct Gene Expression Profiles among Different Histologic Types of Endometrial Cancer Cancer Res., January 1, 2003; 63(1): 6 - 11. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G L Mutter Diagnosis of premalignant endometrial disease J. Clin. Pathol., May 1, 2002; 55(5): 326 - 331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, W. Douglas, M. Lia, W. Edelmann, R. Kucherlapati, K. Podsypanina, R. Parsons, and L. H. Ellenson DNA Mismatch Repair Deficiency Accelerates Endometrial Tumorigenesis in Pten Heterozygous Mice Am. J. Pathol., April 1, 2002; 160(4): 1481 - 1486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-P. Zhou, S. Kuismanen, M. Nystrom-Lahti, P. Peltomaki, and C. Eng Distinct PTEN mutational spectra in hereditary non-polyposis colon cancer syndrome-related endometrial carcinomas compared to sporadic microsatellite unstable tumors Hum. Mol. Genet., February 1, 2002; 11(4): 445 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Hale, C. L. Hughes, and J. M. Cline Endometrial Cancer: Hormonal Factors, the Perimenopausal ""Window of Risk,"" and Isoflavones J. Clin. Endocrinol. Metab., January 1, 2002; 87(1): 3 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Dicuonzo, S. Angeletti, J. Garcia-Foncillas, A. Brugarolas, Y. Okrouzhnov, D. Santini, G. Tonini, G. Lorino, M. De Cesaris, and A. Baldi Colorectal Carcinomas and PTEN/MMAC1 Gene Mutations Clin. Cancer Res., December 1, 2001; 7(12): 4049 - 4053. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Utsunomiya, T. Suzuki, C. Kaneko, J. Takeyama, J. Nakamura, K. Kimura, M. Yoshihama, N. Harada, K. Ito, R. Konno, et al. The Analyses of 17{beta}-Hydroxysteroid Dehydrogenase Isozymes in Human Endometrial Hyperplasia and Carcinoma J. Clin. Endocrinol. Metab., July 1, 2001; 86(7): 3436 - 3443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhu, C.-H. Kwon, P. W. Schlosshauer, L. H. Ellenson, and S. J. Baker PTEN Induces G1 Cell Cycle Arrest and Decreases Cyclin D3 Levels in Endometrial Carcinoma Cells Cancer Res., June 1, 2001; 61(11): 4569 - 4575. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kanamori, J. Kigawa, H. Itamochi, M. Shimada, M. Takahashi, S. Kamazawa, S. Sato, R. Akeshima, and N. Terakawa Correlation between Loss of PTEN Expression and Akt Phosphorylation in Endometrial Carcinoma Clin. Cancer Res., April 1, 2001; 7(4): 892 - 895. [Abstract] [Full Text]
|
|
|
|
|
|
N. Sato, H. Tsunoda, M. Nishida, Y. Morishita, Y. Takimoto, T. Kubo, and M. Noguchi Loss of Heterozygosity on 10q23.3 and Mutation of the Tumor Suppressor Gene PTEN in Benign Endometrial Cyst of the Ovary: Possible Sequence Progression from Benign Endometrial Cyst to Endometrioid Carcinoma and Clear Cell Carcinoma of the Ovary Cancer Res., December 1, 2000; 60(24): 7052 - 7056. [Abstract] [Full Text]
|
|
|
|
|
|
G. L. Maxwell, J. I. Risinger, K. A. Hayes, A. A. Alvarez, R. K. Dodge, J. C. Barrett, and A. Berchuck Racial Disparity in the Frequency of PTEN Mutations, but not Microsatellite Instability, in Advanced Endometrial Cancers Clin. Cancer Res., August 1, 2000; 6(8): 2999 - 3005. [Abstract] [Full Text]
|
|
|
|
|
|
A. H. Holway, K. M. Rieger-Christ, W. R. Miner, J. W. Cain, J. M. Dugan, J. A. Pezza, M. L. Silverman, A. Shapter, R. McLellan, and I. C. Summerhayes Somatic Mutation of PTEN in Vulvar Cancer Clin. Cancer Res., August 1, 2000; 6(8): 3228 - 3235. [Abstract] [Full Text]
|
|
|
|
 |
|
 I. U. Ali Gatekeeper for Endometrium: the PTEN Tumor Suppressor Gene J Natl Cancer Inst, June 7, 2000; 92(11): 861 - 863. [Full Text] [PDF]
|
|
|
|
|
|
G. L. Mutter, M.-C. Lin, J. T. Fitzgerald, J. B. Kum, J. P. A. Baak, J. A. Lees, L.-P. Weng, and C. Eng Altered PTEN Expression as a Diagnostic Marker for the Earliest Endometrial Precancers J Natl Cancer Inst, June 7, 2000; 92(11): 924 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. L. Mutter, M.-C. Lin, J. T. Fitzgerald, J. B. Kum, and C. Eng Changes in Endometrial PTEN Expression throughout the Human Menstrual Cycle J. Clin. Endocrinol. Metab., June 1, 2000; 85(6): 2334 - 2338. [Abstract] [Full Text]
|
|
|
|
|
|
M. Kiechle, M. Hinrichs, A. Jacobsen, J. Luttges, J. Pfisterer, F. Kommoss, and N. Arnold Genetic Imbalances in Precursor Lesions of Endometrial Cancer Detected by Comparative Genomic Hybridization Am. J. Pathol., June 1, 2000; 156(6): 1827 - 1833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Matias-Guiu, E. Bussaglia, L. Catasus, H. Lagarda, E. Gras, P. Machin, J. Prat, W. M. Lin, and C. Y. Muller Correspondence re: W. M. Lin et al., Loss of Heterozygosity and Mutational Analysis of the PTEN/MMAC1 Gene in Synchronous Endometrial and Ovarian Carcinomas. Clin. Cancer Res., 4: 2577-2583, 1998. Clin. Cancer Res., April 1, 2000; 6(4): 1598 - 1600. [Full Text]
|
|
|
|
|
|
I. U. Ali, L. M. Schriml, and M. Dean Mutational Spectra of PTEN/MMAC1 Gene: a Tumor Suppressor With Lipid Phosphatase Activity J Natl Cancer Inst, November 17, 1999; 91(22): 1922 - 1932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Esteller, L. Catasus, X. Matias-Guiu, G. L. Mutter, J. Prat, S. B. Baylin, and J. G. Herman hMLH1 Promoter Hypermethylation Is an Early Event in Human Endometrial Tumorigenesis Am. J. Pathol., November 1, 1999; 155(5): 1767 - 1772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Perren, L.-P. Weng, A. H. Boag, U. Ziebold, K. Thakore, P. L. M. Dahia, P. Komminoth, J. A. Lees, L. M. Mulligan, G. L. Mutter, et al. Immunohistochemical Evidence of Loss of PTEN Expression in Primary Ductal Adenocarcinomas of the Breast Am. J. Pathol., October 1, 1999; 155(4): 1253 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. McMenamin, P. Soung, S. Perera, I. Kaplan, M. Loda, and W. R. Sellers Loss of PTEN Expression in Paraffin-embedded Primary Prostate Cancer Correlates with High Gleason Score and Advanced Stage Cancer Res., September 1, 1999; 59(17): 4291 - 4296. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-M. Georgescu, K. H. Kirsch, T. Akagi, T. Shishido, and H. Hanafusa The tumor-suppressor activity of PTEN is regulated by its carboxyl-terminal region PNAS, August 31, 1999; 96(18): 10182 - 10187. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
