| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Metastasis Research Program, Karmanos Cancer Institute, Detroit, Michigan 48201 [Y. N., S. P., A. R., A. H.]; Department of Orthopedic Surgery, Gunma University School of Medicine, Gunma, 371, Japan [H. W.]; and Department of Pathology and Radiation Oncology, Wayne State University School of Medicine, Detroit, Michigan 48202 [A. R.]
The results obtained from fragmented protein microsequencing have suggested that autocrine motility factor (AMF), a tumor-secreted Mr 55,000 cytokine that regulates cell motility in vitro as well as invasion and metastasis in vivo, is the neuroleukin (NLK)/phosphohexose isomerase (PHI)/maturation factor (MF) polypeptide. Here, we cloned, sequenced, and studied the expression, secretion, and distribution of AMF/NLK/PHI/MF in neoplastic and their normal counterpart cells. Although both normal and neoplastic cells express the gene product, overexpression associated with selective secretion of the protein was observed only in tumor cells. The cDNA sequences of AMF/NLK/PHI/MF found in both human cancer and normal cells were found to be identical, suggesting that its secretion by neoplastic cells is independent of mutation or alternative splicing. Immunohistochemical visualization has depicted AMF/NLK/PHI/MF to be localized into tubular-like vesicles, diffusely distributed throughout the cytoplasm and not colocalized with any particular cytoskeletal network. Confocal microscopic imaging had shown a partial colocalization between AMF and its receptor (Mr 78,000 glycoprotein), especially on the malignant cell surface periphery. The results suggest that extracellular AMF activity may be a result of the product of intracellular cleavage of a precursor polypeptide, which is overexpressed and selectively secreted through a nonclassical secretory mechanism by neoplastic cells.
1 This work was supported in part by NIH Grant R01-CA51714 and by the Paul Zuckerman Support Foundation for Cancer Research (to A. R.).
2 To whom requests for reprints should be addressed, at Metastasis Research Program, Karmanos Cancer Institute, 110 East Warren, Detroit, MI 48201. Phone: (313) 833-0715; Fax: (313) 831-7518; E-mail: raza@kci.wayne.edu.
Received 1/22/98. Accepted 4/20/98.
This article has been cited by other articles:
|
|
 |
|
  T. Funasaka, H. Hu, V. Hogan, and A. Raz Down-regulation of Phosphoglucose Isomerase/Autocrine Motility Factor Expression Sensitizes Human Fibrosarcoma Cells to Oxidative Stress Leading to Cellular Senescence J. Biol. Chem., December 14, 2007; 282(50): 36362 - 36369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Funasaka, H. Hu, T. Yanagawa, V. Hogan, and A. Raz Down-Regulation of Phosphoglucose Isomerase/Autocrine Motility Factor Results in Mesenchymal-to-Epithelial Transition of Human Lung Fibrosarcoma Cells Cancer Res., May 1, 2007; 67(9): 4236 - 4243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. L. Decker and W. T. Wickner Enolase Activates Homotypic Vacuole Fusion and Protein Transport to the Vacuole in Yeast J. Biol. Chem., May 19, 2006; 281(20): 14523 - 14528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P M Candler, R C Dale, S Griffin, A J Church, R Wait, M D Chapman, G Keir, G Giovannoni, and J H Rees Post-streptococcal opsoclonus-myoclonus syndrome associated with anti-neuroleukin antibodies J. Neurol. Neurosurg. Psychiatry, April 1, 2006; 77(4): 507 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. F. Shih, J. Racz, and P. M. Allen Differential MHC Class II Presentation of a Pathogenic Autoantigen during Health and Disease J. Immunol., March 15, 2006; 176(6): 3438 - 3448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Jiang, A. Raz, A. Douglas-Jones, and R. E. Mansel Expression of Autocrine Motility Factor (AMF) and Its Receptor, AMFR, in Human Breast Cancer J. Histochem. Cytochem., February 1, 2006; 54(2): 231 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Funasaka, T. Yanagawa, V. Hogan, and A. Raz Regulation of phosphoglucose isomerase/autocrine motility factor expression by hypoxia FASEB J, September 1, 2005; 19(11): 1422 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T Yanagawa, T Funasaka, S Tsutsumi, H Watanabe, and A Raz Novel roles of the autocrine motility factor/phosphoglucose isomerase in tumor malignancy Endocr. Relat. Cancer, December 1, 2004; 11(4): 749 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tsutsumi, T. Yanagawa, T. Shimura, H. Kuwano, and A. Raz Autocrine Motility Factor Signaling Enhances Pancreatic Cancer Metastasis Clin. Cancer Res., November 15, 2004; 10(22): 7775 - 7784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. O-charoenrat, V. Rusch, S. G. Talbot, I. Sarkaria, A. Viale, N. Socci, I. Ngai, P. Rao, and B. Singh Casein Kinase II Alpha Subunit and C1-Inhibitor Are Independent Predictors of Outcome in Patients with Squamous Cell Carcinoma of the Lung Clin. Cancer Res., September 1, 2004; 10(17): 5792 - 5803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Biarc, I. S. Nguyen, A. Pini, F. Gosse, S. Richert, D. Thierse, A. Van Dorsselaer, E. Leize-Wagner, F. Raul, J.-P. Klein, et al. Carcinogenic properties of proteins with pro-inflammatory activity from Streptococcus infantarius (formerly S.bovis) Carcinogenesis, August 1, 2004; 25(8): 1477 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mishra, A. Raz, and L. J. Murphy Insulin-Like Growth Factor Binding Protein-3 Interacts with Autocrine Motility Factor/Phosphoglucose Isomerase (AMF/PGI) and Inhibits the AMF/PGI Function Cancer Res., April 1, 2004; 64(7): 2516 - 2522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tsutsumi, T. Yanagawa, T. Shimura, T. Fukumori, V. Hogan, H. Kuwano, and A. Raz Regulation of Cell Proliferation by Autocrine Motility Factor/Phosphoglucose Isomerase Signaling J. Biol. Chem., August 22, 2003; 278(34): 32165 - 32172. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. C. Schulz and J. M. Bahr Glucose-6-phosphate isomerase is necessary for embryo implantation in the domestic ferret PNAS, July 8, 2003; 100(14): 8561 - 8566. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Prosniak, A. Zborek, G. S. Scott, A. Roy, T. W. Phares, H. Koprowski, and D. C. Hooper Differential expression of growth factors at the cellular level in virus-infected brain PNAS, May 27, 2003; 100(11): 6765 - 6770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Krishnamachary, S. Berg-Dixon, B. Kelly, F. Agani, D. Feldser, G. Ferreira, N. Iyer, J. LaRusch, B. Pak, P. Taghavi, et al. Regulation of Colon Carcinoma Cell Invasion by Hypoxia-Inducible Factor 1 Cancer Res., March 1, 2003; 63(5): 1138 - 1143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tsutsumi, V. Hogan, I. R. Nabi, and A. Raz Overexpression of the Autocrine Motility Factor/Phosphoglucose Isomerase Induces Transformation and Survival of NIH-3T3 Fibroblasts Cancer Res., January 1, 2003; 63(1): 242 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Talukder, R. Bagheri-Yarmand, R. R. E. Williams, J. Ragoussis, R. Kumar, and A. Raz Antihuman Epidermal Growth Factor Receptor 2 Antibody Herceptin Inhibits Autocrine Motility Factor (AMF) Expression and Potentiates Antitumor Effects of AMF Inhibitors Clin. Cancer Res., October 1, 2002; 8(10): 3285 - 3289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tsutsumi, S. K. Gupta, V. Hogan, J. G. Collard, and A. Raz Activation of Small GTPase Rho Is Required for Autocrine Motility Factor Signaling Cancer Res., August 1, 2002; 62(15): 4484 - 4490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Hellmann, W. R. Fields, and D. J. Doolittle Gene Expression Profiling of Cultured Human Bronchial Epithelial and Lung Carcinoma Cells Toxicol. Sci., May 1, 2001; 61(1): 154 - 163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Yakirevich and Y. Naot Cloning of a Glucose Phosphate Isomerase/Neuroleukin-Like Sperm Antigen Involved in Sperm Agglutination Biol Reprod, April 1, 2000; 62(4): 1016 - 1023. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. Le, N Benlimame, A Lagana, A Raz, and I. Nabi Clathrin-mediated endocytosis and recycling of autocrine motility factor receptor to fibronectin fibrils is a limiting factor for NIH-3T3 cell motility J. Cell Sci., January 9, 2000; 113(18): 3227 - 3240. [Abstract] [PDF]
|
|
|
|
|
|
A. H. Talukder, L. Adam, A. Raz, and R. Kumar Heregulin Regulation of Autocrine Motility Factor Expression in Human Tumor Cells Cancer Res., January 1, 2000; 60(2): 474 - 480. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-J. Sun, C.-C. Chou, W.-S. Chen, R.-T. Wu, M. Meng, and C.-D. Hsiao The crystal structure of a multifunctional protein: Phosphoglucose isomerase/autocrine motility factor/neuroleukin PNAS, May 11, 1999; 96(10): 5412 - 5417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Chou, Y.-J. Sun, M. Meng, and C.-D. Hsiao The Crystal Structure of Phosphoglucose Isomerase/Autocrine Motility Factor/Neuroleukin Complexed with Its Carbohydrate Phosphate Inhibitors Suggests Its Substrate/Receptor Recognition J. Biol. Chem., July 21, 2000; 275(30): 23154 - 23160. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
