| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan 115 [K. P., Y-P. S., S. R. R., C-W. W., P-C. Y.]; and Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan 100 [J-Y. S., P-C. Y.], Republic of China
Detection and quantitation of circulating cancer cells in peripheral blood may improve cancer staging and monitoring. This study explored the feasibility of using circulating cancer cell detection in peripheral blood for the rapid assessment of chemotherapeutic response. Cytokeratin 19 mRNA was amplified by nested reverse transcriptase-PCR in the peripheral blood of 29 healthy volunteers, 33 pneumonia patients, and 86 lung cancer patients. Circulating cancer cells in the peripheral blood were semiquantitatively determined by taking the ratio of cytokeratin 19 band intensity from the second round of nested PCR to the glyceraldehyde-3-phosphate dehydrogenase band intensity from the first round of PCR amplification. The detection limit of the method was 1 cancer cell in 107 peripheral blood mononuclear cells. The positive detection rate was 40% for lung adenocarcinoma patients of all stages, 41% for squamous carcinoma patients of all stages, and 27% for small cell lung cancer patients. Only one control sample from a pneumonia patient showed a positive result (1.6%). The quantitative method reliably and sensitively estimated cancer cell numbers in the peripheral blood of lung cancer patients. Serial measurement of the relative number of circulating cancer cells correlated with the tumor burden and treatment response of patients. This method may help rapidly assess the efficacy of anticancer treatment, redefine cancer staging, and facilitate the design of better therapeutic strategies for the treatment of cancer patients.
1 This work was supported by Metastasis Program Project grant from Academia Sinica (Taipei, Taiwan, Republic of China).
2 To whom requests for reprints should be addressed.
Received 3/ 4/98. Accepted 4/27/98.
This article has been cited by other articles:
|
|
 |
|
  T. Yamabuki, A. Takano, S. Hayama, N. Ishikawa, T. Kato, M. Miyamoto, T. Ito, H. Ito, Y. Miyagi, H. Nakayama, et al. Dikkopf-1 as a Novel Serologic and Prognostic Biomarker for Lung and Esophageal Carcinomas Cancer Res., March 15, 2007; 67(6): 2517 - 2525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-P. Sher, C.-C. Chou, R.-H. Chou, H.-M. Wu, W.-S. Wayne Chang, C.-H. Chen, P.-C. Yang, C.-W. Wu, C.-L. Yu, and K. Peck Human Kallikrein 8 Protease Confers a Favorable Clinical Outcome in Non-Small Cell Lung Cancer by Suppressing Tumor Cell Invasiveness Cancer Res., December 15, 2006; 66(24): 11763 - 11770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Pathak, M. Bhutani, S. Kumar, A. Mohan, and R. Guleria Circulating Cell-Free DNA in Plasma/Serum of Lung Cancer Patients as a Potential Screening and Prognostic Tool Clin. Chem., October 1, 2006; 52(10): 1833 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ramnath, E. Sommers, L. Robinson, C. Nwogu, A. Sharma, A. Cantor, and G. Bepler Phase II Study of Neoadjuvant Chemotherapy With Gemcitabine and Vinorelbine in Resectable Non-small Cell Lung Cancer Chest, November 1, 2005; 128(5): 3467 - 3474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-P. Sher, J.-Y. Shih, P.-C. Yang, S. R. Roffler, Y.-W. Chu, C.-W. Wu, C.-L. Yu, and K. Peck Prognosis of Non-Small Cell Lung Cancer Patients by Detecting Circulating Cancer Cells in the Peripheral Blood with Multiple Marker Genes Clin. Cancer Res., January 1, 2005; 11(1): 173 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mitas, L. Hoover, G. Silvestri, C. Reed, M. Green, A. T. Turrisi, C. Sherman, K. Mikhitarian, D. J. Cole, M. I. Block, et al. Lunx Is a Superior Molecular Marker for Detection of Non-Small Lung Cell Cancer in Peripheral Blood J. Mol. Diagn., November 1, 2003; 5(4): 237 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mitas, D. J. Cole, L. Hoover, M. M. Fraig, K. Mikhitarian, M. I. Block, B. J. Hoffman, R. H. Hawes, W. E. Gillanders, and M. B. Wallace Real-Time Reverse Transcription-PCR Detects KS1/4 mRNA in Mediastinal Lymph Nodes from Patients with Non-Small Cell Lung Cancer Clin. Chem., February 1, 2003; 49(2): 312 - 315. [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Quarterman, A. McMillan, M. B. Ratcliffe, and M. I. Block Effect of preoperative delay on prognosis for patients with early stage non-small cell lung cancer J. Thorac. Cardiovasc. Surg., January 1, 2003; 125(1): 108 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lillo, M. Ramirez, A. Alvarez, S. Santos, J. Garcia-Castro, J. Fernandez de Velasco, M. J. Aviles, A. Gomez-Pineda, J. L. Diez, A. Balas, et al. Efficient and Nontoxic Adenoviral Purging Method for Autologous Transplantation in Breast Cancer Patients Cancer Res., September 1, 2002; 62(17): 5013 - 5018. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Stathopoulou, I. Vlachonikolis, D. Mavroudis, M. Perraki, Ch. Kouroussis, S. Apostolaki, N. Malamos, S. Kakolyris, A. Kotsakis, N. Xenidis, et al. Molecular Detection of Cytokeratin-19-Positive Cells in the Peripheral Blood of Patients With Operable Breast Cancer: Evaluation of Their Prognostic Significance J. Clin. Oncol., August 15, 2002; 20(16): 3404 - 3412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Jiao and M. J. Krasna Clinical significance of micrometastasis in lung and esophageal cancer: a new paradigm in thoracic oncology Ann. Thorac. Surg., July 1, 2002; 74(1): 278 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J R C Ellis and F V Gleeson Lung cancer screening Br. J. Radiol., June 1, 2001; 74(882): 478 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. F. Patz, P. C. Goodman, and G. Bepler Screening for Lung Cancer N. Engl. J. Med., November 30, 2000; 343(22): 1627 - 1633. [Full Text] [PDF]
|
|
|
|
|
|
T. Hashimoto, Y. Kobayashi, Y. Ishikawa, S. Tsuchiya, S. Okumura, K. Nakagawa, Y. Tokuchi, M. Hayashi, K. Nishida, S.-i. Hayashi, et al. Prognostic Value of Genetically Diagnosed Lymph Node Micrometastasis in Non-Small Cell Lung Carcinoma Cases Cancer Res., November 1, 2000; 60(22): 6472 - 6478. [Abstract] [Full Text]
|
|
|
|
|
|
V. M. M. van Houten, M. P. Tabor, M. W. M. van den Brekel, F. Denkers, R. G. A. Wishaupt, J. A. Kummer, G. B. Snow, and R. H. Brakenhoff Molecular Assays for the Diagnosis of Minimal Residual Head-and-Neck Cancer: Methods, Reliability, Pitfalls, and Solutions Clin. Cancer Res., October 1, 2000; 6(10): 3803 - 3816. [Abstract] [Full Text]
|
|
|
|
|
|
E. F. Patz Jr., S. Rossi, D. H. Harpole Jr., J. E. Herndon, and P. C. Goodman Correlation of Tumor Size and Survival in Patients With Stage IA Non-small Cell Lung Cancer Chest, June 1, 2000; 117(6): 1568 - 1571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Wyckoff, J. G. Jones, J. S. Condeelis, and J. E. Segall A Critical Step in Metastasis: In Vivo Analysis of Intravasation at the Primary Tumor Cancer Res., May 1, 2000; 60(9): 2504 - 2511. [Abstract] [Full Text]
|
|
|
|
|
|
B. M. Smith, M. J. Slade, J. English, H. Graham, M. Luchtenborg, H. D. Sinnett, N. C. P. Cross, and R. C. Coombes Response of Circulating Tumor Cells to Systemic Therapy in Patients With Metastatic Breast Cancer: Comparison of Quantitative Polymerase Chain Reaction and Immunocytochemical Techniques J. Clin. Oncol., April 7, 2000; 18(7): 1432 - 1439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Morifuji, S.’i. Taniguchi, H. Sakai, Y. Nakabeppu, and M. Ohishi Differential Expression of Cytokeratin after Orthotopic Implantation of Newly Established Human Tongue Cancer Cell Lines of Defined Metastatic Ability Am. J. Pathol., April 1, 2000; 156(4): 1317 - 1326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhang, S. Kharbanda, S. W. McLeskey, and F. G. Kern Overexpression of Fibroblast Growth Factor 1 in MCF-7 Breast Cancer Cells Facilitates Tumor Cell Dissemination but Does Not Support the Development of Macrometastases in the Lungs or Lymph Nodes Cancer Res., October 1, 1999; 59(19): 5023 - 5029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Fournier, F. C. Guimaraes, M. E. M. Paschoal, L. V. Ronco, M. d. G. C. Carvalho, and A. B. Pardee Identification of a Gene Encoding a Human Oxysterol-binding Protein-Homologue: A Potential General Molecular Marker for Blood Dissemination of Solid Tumors Cancer Res., August 1, 1999; 59(15): 3748 - 3753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. I. Garver, G. L. Zorn, X. Wu, D. C. McGiffin, K. R. Young, and N. B. Pinkard Recurrence of Bronchioloalveolar Carcinoma in Transplanted Lungs N. Engl. J. Med., April 8, 1999; 340(14): 1071 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. F. Patz Jr, W. C. Black, and P. C. Goodman CT Screening for Lung Cancer: Not Ready for Routine Practice Radiology, December 1, 2001; 221(3): 587 - 591. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
